To further our understanding of powdery mildew biology during infection, we undertook a systematic shotgun proteomics analysis of the obligate biotroph Blumeria graminis f. sp. hordei at different stages of development in the host. Moreover we used a proteogenomics approach to feed information into the annotation of the newly sequenced genome. We analyzed and compared the proteomes from three s...

Durable disease resistance genes such as the wheat gene Lr34 are valuable sources of for agricultural breeding programs. encodes an ATP-binding cassette transporter protein involved in transport phytohormone abscisic acid. from is functionally transferable to barley, maize, rice and sorghum. A pleiotropic effect induces development a senescence-like phenotype, referred leaf tip necrosis. We use...

Pathogenic microbes manipulate eukaryotic cells during invasion and target plant proteins to achieve host susceptibility. BAX INHIBITOR-1 (BI-1) is an endoplasmic reticulum-resident cell death suppressor in plants and animals and is required for full susceptibility of barley to the barley powdery mildew fungus Blumeria graminis f.sp. hordei. LIFEGUARD (LFG) proteins resemble BI-1 proteins in te...

Little is known about the function of host factors involved in disease susceptibility. The barley (Hordeum vulgare) ROP (RHO of plants) G-protein RACB is required for full susceptibility of the leaf epidermis to invasion by the biotrophic fungus Blumeria graminis f. sp hordei. Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epi...

Small GTP-binding proteins such as those from the RAC family are cytosolic signal transduction proteins that often are involved in processing of extracellular stimuli. Plant RAC proteins are implicated in regulation of plant cell architecture, secondary wall formation, meristem signaling, and defense against pathogens. We isolated a RacB homolog from barley (Hordeum vulgare) to study its role i...

Abstract The powdery mildew fungi secrete numerous Candidate Secreted Effector Proteins (CSEPs) to manipulate host immunity during infection of plants. However, the function most these CSEPs in cell death suppression has not yet been established. Here, we identified several from Blumeria graminis f. sp. hordei ( Bgh ) that have potential suppress BAX- and Nt MEK2 DD -triggered Nicotiana bentham...

Methanol extracts from 27 medicinal plant species were tested at concentrations of 0.5, 1 and 2 mg/mL for their in vivo fungicidal activities against six phytopathogenic fungi. Their efficacy varied with plant pathogen, tissue sampled and plant species. Very strong fungicidal activity was produced by extracts of Boswellia carterii, Saussurea lappa, Glycyrrhiza uralensis, Piper nigrum, Rheum cor...

cDNA microarrays of Blumeria graminis f sp hordei transcript profiles during the asexual development cycle reveal the dynamics of global gene expression as the fungus germinates, penetrates, feeds on its host, and produces masses of conidia for dispersal. The expression profiles of genes encoding enzymes involved in primary metabolism show that there is a striking degree of coordinate regulatio...