The application of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system of Streptococcus pyogenes (SpCas9) is currently revolutionizing genome engineering in plants. However, synthetic plant biology will require more complex manipulations of genomes and transcriptomes. The simultaneous addressing of different specific genomic sites with independent enzyme activities...

Genome targeting of restriction enzymes and DNA methyltransferases has many important applications including genome and epigenome editing. 15-20 years ago, my group was involved in the development of approaches for programmable genome targeting, aiming to connect enzymes with an oligodeoxynucleotide (ODN), which could form a sequence-specific triple helix at the genomic target site. Importantly...

The protein tyrosine phosphatase PTP-PEST is a cytosolic enzyme that displays a remarkable degree of selectivity for tyrosine-phosphorylated p130(Cas) as a substrate, both in vitro and in intact cells. We have investigated the physiological role of PTP-PEST using Rat1 fibroblast-derived stable cell lines that we have engineered to overexpress PTP-PEST. These cell lines exhibit normal levels of ...

Background: Carotid Artery Stenosis (CAS) is one of the major high-risk mechanisms of stroke. Neopterin, an immune modulator secreted by macrophages, have been reported to be elevated in patients with cerebrovascular disease. However, an association between serum neopterin level and CAS has not been firmly established. Objective: To investigate the cross-sectional relationship between serum neo...

We investigated the catalytic activity and inhibition of the delta-class carbonic anhydrase (CA, EC 4.2.1.1) from the marine diatom Thalassiosira weissflogii, TweCA. The enzyme, obtained by cloning the synthetic gene, was an efficient catalyst for the CO2 hydration, its physiological reaction, with a kcat of 1.3x105s-1 and a kcat/KM of 3.3x107M-1s-1. A range of inorganic anions and small molecu...

Les auteurs rapportent chez deux nourrissons, l'etude anatomo-clinique d'un cas de kystes alveolaires multiples,et d'un cas d'emphyseme lobaire congenital bilateral.Le troisieme cas concerne un kyste bronchog6nique du poumon gauche,decouvert a l'autopsie d'un nouveau-ne. Dans le quatrieme cas,un emphyseme geant du lobe superieur gauche,decouvert dans un contexte infectieux,a l'age de neuf mois,...

conclusions presence of cae in patients with cas increases its cardiovascular event rate. isolated cae is not a benign finding and mi can occur. risk factors of cae are similar to cas, but c/s is more associated with cae than cas. dm is seen in cae patients less than cas. results hypertension (htn), dyslipidemia (dlp), and male sex were matched in both groups without significant difference. cig...

A series of novel isatin-linked 4,4-dimethyl-5-methylene-4,5-dihydrothiazole-2-thiols (IT2Ts) 1a–1g were designed as acetylcholinesterase (AChE) inhibitors capable interacting with both the catalytic active site (CAS) and peripheral anionic (PAS) enzyme simultaneously. The target IT2Ts prepared through a short synthesis in moderate yield. most potent this 1b 1c (IC50 = 18.2 27.5 μM, respectivel...

It has been reported that individuals with the D allele of an insertion/deletion (I/D) polymorphism of the angiotensin converting enzyme (ACE) gene are at greater risk for myocardial infarction (MI), especially among subjects normally considered to be at low risk. However, little is known about the mechanism by which the ACE polymorphism affects the risk of MI. Coronary artery spasm (CAS) is co...

background: carotid artery stenting (cas) has recently been recommended as an alternative to carotid endarterectomy (cea) by some clinicians. objective: this study was designed to evaluate the success rate and in-hospital and 30-day adverse events in our first experiences in iran for cas with protection devices, to document our results and guide further use of cas. methods: from december 2003 t...