نتایج جستجو برای: capture elisa

تعداد نتایج: 163920  

Journal: :Annals of clinical and laboratory science 1991
K Doetsch P S Roheim J J Thompson

Plasma lipoprotein(a), Lp(a), is the most important known genetically controlled independent risk factor for the prediction of early atherosclerosis (AS) and coronary artery disease (CAD) in a significant subpopulation of Caucasians. A sensitive, specific 'capture' enzyme linked immunosorbent assay (ELISA) is reported for the assay of human plasma Lp(a). There is no interference from low densit...

2007
Bahador Sarkari Michael Chance Marcel Hommel

A capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from ...

2016
Aiko Fukuma Shuetsu Fukushi Tomoki Yoshikawa Hideki Tani Satoshi Taniguchi Takeshi Kurosu Kazutaka Egawa Yuto Suda Harpal Singh Taro Nomachi Mutsuyo Gokuden Katsuyuki Ando Kouji Kida Miki Kan Nobuyuki Kato Akira Yoshikawa Hiroaki Kitamoto Yuko Sato Tadaki Suzuki Hideki Hasegawa Shigeru Morikawa Masayuki Shimojima Masayuki Saijo

BACKGROUND Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease with a high case fatality rate, and is caused by the SFTS virus (SFTSV). SFTS is endemic to China, South Korea, and Japan. The viral RNA level in sera of patients with SFTS is known to be strongly associated with outcomes. Virological SFTS diagnosis with high sensitivity and specificity are required...

Journal: :Biochemistry. Biokhimiia 1999
V A Nikulina E A Kizim Y S Massino O L Segal M B Smirnova V V Avilov D B Saprygin S P Smotrov G I Kolyaskina A D Dmitriev

Using a panel of monoclonal antibodies against human myoglobin (Mb), we have shown that the sensitivity of antigen-capture ELISA can be significantly increased by simultaneous immobilization of two cooperating capture monoclonal antibodies on a solid phase. This method ("triple-site ELISA") uses three monoclonal antibodies to different epitopes of the same antigen (two capture/one tracer) unlik...

Journal: :Clinical and diagnostic laboratory immunology 2003
Mårten Segelmark Brian D Phillips Susan L Hogan Ronald J Falk J Charles Jennette

The clinical usefulness of antineutrophil cytoplasmic antibodies (ANCAs) in the monitoring of patients treated for small vessel vasculitis is debated. A capture enzyme-linked immunosorbent assay (ELISA) based on anti-proteinase 3 (anti-PR3) monoclonal antibody 4A3 has previously been proven to be superior to indirect immunofluorescence (IIF) and standard ELISA for the diagnosis of vasculitis. T...

Journal: :Advances in Animal and Veterinary Sciences 2015

Journal: :Journal of immunological methods 2001
M M Boomsma C A Stegeman W W Oost-Kort C G Kallenberg N Moguilevsky P C Limburg J W Tervaert

The prevalence of Anti-Neutrophil Cytoplasmic Antibodies (ANCA) directed against myeloperoxidase (MPO) in pauci-immune necrotizing crescentic glomerulonephritis (NCGN) is dependent on the assay(s) used. We investigated the frequency of MPO-ANCA as detected by different assays for MPO-ANCA in a large cohort of patients with biopsy-proven pauci-immune NCGN. Sera from 121 consecutive untreated pat...

Journal: :Clinical and vaccine immunology : CVI 2007
Mindy L Rawlins Erica M Swenson Harry R Hill Christine M Litwin

Since the introduction of West Nile virus (WNV) in the United States in 1999, several assays have become commercially available to detect antibodies against WNV. Capture enzyme-linked immunosorbent assays (ELISAs) for the detection of WNV-specific immunoglobulin M (IgM) have been approved by the Food and Drug Administration for clinical testing and are available from Focus Diagnostics and PanBi...

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