Several in vitro assays have been developed to evaluate the gastrointestinal absorption of compounds. Our aim was to compare 3 of these methods: 1) the bio-mimetic artificial membrane permeability assay (BAMPA) method, which offers a high-throughput, noncellular approach to the measurement of passive transport; 2) the traditional Caco-2 cell assay, the use of which as a high-throughput tool is ...

Ma, Thomas Y., Michel A. Boivin, Dongmei Ye, Ali Pedram, and Hamid M. Said. Mechanism of TNFmodulation of Caco-2 intestinal epithelial tight junction barrier: role of myosin light-chain kinase protein expression. Am J Physiol Gastrointest Liver Physiol 288: G422–G430, 2005; doi:10.1152/ajpgi.00412.2004.—TNFplays a central role in the intestinal inflammation of various inflammatory disorders inc...

Ma, Thomas Y., Gary K. Iwamoto, Neil T. Hoa, Vimesh Akotia, Ali Pedram, Michel A. Boivin, and Hamid M. Said. TNFinduced increase in intestinal epithelial tight junction permeability requires NFB activation. Am J Physiol Gastrointest Liver Physiol 286: G367–G376, 2004; 10.1152/ajpgi.00173.2003.—Crohn’s disease (CD) patients have an abnormal increase in intestinal epithelial permeability. The def...

TNF-alpha plays a central role in the intestinal inflammation of various inflammatory disorders including Crohn's disease (CD). TNF-alpha-induced increase in intestinal epithelial tight junction (TJ) permeability has been proposed as one of the proinflammatory mechanisms contributing to the intestinal inflammation. The intracellular mechanisms involved in the TNF-alpha-induced increase in intes...

The uptake and transport of 9-nitrocamptothecin (9-NC), a potent anticancer agent, across Caco-2 cell monolayers was studied as a free and PLGA nanoparticle loaded drug. Different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. The transport of nanoparticles across the Caco-2 cell monolayer as a func...

The uptake and transport of 9-nitrocamptothecin (9-NC), a potent anticancer agent, across Caco-2 cell monolayers was studied as a free and PLGA nanoparticle loaded drug. Different sizes (110 to 950 nm) of 9-nitrocamptothecin nanoparticles using poly (lactic-glycolic acid) were prepared by via the nanoprecipitation method. The transport of nanoparticles across the Caco-2 cell monolayer as a func...