Brucellosis is a zoonotic disease with veterinary, public health, and economic implications. The study aimed to estimate the seroprevalence of Brucella spp. among ruminants in Rafha, Saudi Arabia during January October 2022 identify camel strains based on glycosyltransferase gene sequence. Sera (n=1012) were collected from non-vaccinated sheep, goats, camels cattle different sex, age breed rand...

Monoclonal antibodies which bind Brucella A lipopolysaccharide (LPS)-specific, M LPS-specific, or cross-reactive epitopes were used as reagents in quantitative dot blot, Western blot (immunoblot), and immunoprecipitation analysis of Brucella whole cells, whole-cell extracts, and purified LPS preparations. This set of monoclonal antibodies detected four unique epitopes on Brucella LPS. The speci...

Brucella spp. is a worldwide zoonotic pathogen. Infection by Brucella canis in dogs is endemic in the Southern USA and in Central and South America, but it appears sporadically in other parts of the world, including Europe. Tissue samples from a dog with chronic prostatitis, discospondylitis and locomotor problems were subjected to clinical and laboratory examinations. B. canis was detected by ...

background: brucellosis is one of the most common zoonosis in middle east and iran. objectives: the purpose of this study was genomic detection of brucella spp. in sero-positive dairy cattle. methods: we have collected 28,519 blood samples from cows during 2012-2013. samples were screened by slide and tube agglutination and 2-mercaptoethanol tests. samples with anti-brucella antibodies titer ≥ ...

Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antig...

Background: Brucellosis is an endemic zoonotic disease in the Middle East. This study intended to design a uniplex PCR assay for the detection and differentiation of Brucella at the species level and determining the antibiotic susceptibility pattern of Brucella in Iran. Methods: Sixty-eight Brucella specimens (38 an...

objective(s): brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. immunity against brucella melitensis involves antigen-specific cd4+ and cd8+ t-cells activation and humoral immune responses. due to negative aspects of live attenuated vaccines, much attention has been focused on finding brucella-protective antig...

This paper reports on a sporadic, naturally acquired infection of sheep with Brucella abortus on a privately owned farm in Toro near Bauchi, Nigeria. The abortions, which occurred in a flock of 28 Yankassa sheep, involved five ewes at the third month of gestation. Serum and milk samples from the flock were examined for Brucella antibodies by the Rose Bengal plate test, serum agglutination test ...

Background: Using Brucella abortus Strain 19 (S19) to control bovine brucellosis is restricted due to induce antibodies to the O-side chain of the smooth lipopolysaccharide (LPS) which may be difficult to differentiate vaccinated and infected animals. Furthermore, it is virulent for humans and can induce abortion to cattle. Objectives: The aim of...

Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not introduced into the U.S. goat population. The objective of this study was to de...