b a ckground: babesiosis is a haemoparasitic disease of domestic and wild animals caused by species of the genus bab esia . bab esia bigemina , b . bovis and b . divergens are known to be pathogenic in cattle. the disease is transmit- ted during blood feeding by infected ticks and is the most economically important tick-borne disease in tropical and subtropical areas. ixodid ticks are vectors i...

background: progress in the field of biology and biochemistry has led to the discovery of numerous bioactive peptides and proteins in the last few decades. delivery of therapeutic proteins/peptides has received a considerable amount of attention in recent years. methods: in this study, a two-step desolvation method was used to produce biodegradable hydrophilic gelatin nanoparticles (gnp) as a d...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...

conclusions in this study, the l-lps immune response of bovine cells was significant, indicating that tlr2 is the predominant receptor for l-lps. due to l-lps endotoxin-like activity, we found a strategy through using bmap-28 to prevent l-lps-induced tlr2-dependent immune activation in bovine cells. materials and methods in this study, l-lps-induced proinflammatory cytokine production in bovine...

testosterone is believed to play a significant role in spermatogenesis, but its contribution to the process of spermatogenesis is not completely understood. given that extracellular matrix (ecm) facilitates differentiation of spermatogonial stem cells (sscs) during culture, the present study was conducted to elucidate whether testosterone contribute to the permissive effect of ecm on sscs diffe...

improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. the aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different precompacted morulae stages by assessment of cryosurvivability of the resulting blastocysts. the in vitr...

background: oocyte maturation and subsequent in vitro production (ivp) of embryos are affected by diverse groups of chemicals in maturation medium which are needed for successful mammalian oocyte maturation during which the dramatic cytoplasmic and nuclear reprogramming events take place. this study was designed to evaluate the effects of protein source (fetal bovine serum, fbs, and bovine seru...

the complex process of spermatogenesis is regulated by various factors. studies on spermatogonial stem cells (sccs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. to investigate and biomanipulation of these cells, proliferation and viability rate of cells should be ...

in this syudy a continuous bovine kidney cell line derived from a primary bovine kidney cells was established for the first time in iran. the cells were originating from two-day-old normal male calf of holstein breed. the cell cultures were continuously passaged following complete proliferation of primary cells. the specific properties or characteristics of the cell were defined using cytogene...