نتایج جستجو برای: biolistics

تعداد نتایج: 273  

2013
Jindřich Bříza Daniela Pavingerová Josef Vlasák Hana Niedermeierová

Modified versions of the Cry3A gene of Bacillus thuringiensis (Bt) were transferred into Norway spruce (Picea abies). Both the biolistic approach and Agrobacterium tumefaciens mediated procedure were employed for transformation of embryogenic tissue (ET) cultures. The latter method proved to be more efficient yielding 70 transgenic embryogenic tissue lines compared with 18 lines obtained by bio...

Journal: :Cold Spring Harbor protocols 2009
David J Cove Pierre-François Perroud Audra J Charron Stuart F McDaniel Abha Khandelwal Ralph S Quatrano

RELATED INFORMATION For more information about P. patens as a model organism, see The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies (Cove et al. 2009a). The growth of protonemal tissue is described in Culturing the Moss Physcomitrella patens (Cove et al. 2009b), and a method for isolation of P. patens protoplasts is found in Isolation and Regeneratio...

2006
Yi Liu Fang Liu

By means of a transient transonic helium jet generated by a convergent-divergent conical nozzle, a unique biolistic method of accelerating microparticle formulations of DNA vaccines or drugs to penetrate the outer layer of human skin or mucosal tissue to elicit an physiological response for the treatment of a range of diseases. In this paper, Computational Fluid Dynamics is utilized to predict ...

Journal: :Journal of virology 2005
Yan-Wei Huang Yun-Feng Geng Xiao-Bao Ying Xiao-Ying Chen Rong-Xiang Fang

Rice yellow stunt rhabdovirus (RYSV) encodes seven genes in its negative-sense RNA genome in the order 3'-N-P-3-M-G-6-L-5'. The existence of gene 3 in the RYSV genome and an analogous gene(s) of other plant rhabdoviruses positioned between the P and M genes constitutes a unique feature for plant rhabdoviruses that is distinct from animal-infecting rhabdoviruses in which the P and M genes are di...

Journal: :Methods in molecular biology 2005
Julie R Kikkert José R Vidal Bruce I Reisch

Particle bombardment, or biolistics, is a commonly used method for genetic transformation of plants and other organisms. Millions of DNA-coated metal particles are shot at target cells or tissues using a biolistic device or gene gun. The DNA elutes off the particles that lodge inside the cells, and a portion may be stably incorporated in the host chromosomes. A protocol for the generation of tr...

Journal: :Methods in molecular biology 2012
Andy C Wetten Jean-Luc Thomas Alina Wagiran Tinashe Chiurugwi

A transgenesis programme has been developed for Impatiens balsamina that will allow elucidation of the roles played by individual genes in the flower reversion phenomenon shown by this model species. The lack of explants exhibiting adventitious shooting in I. balsamina hinders Agrobacterium-based transformation, but the multiple shoots that arise from cotyledonary nodes present a suitable targe...

Journal: :BioTechniques 2002
Terrance T Kummer Hiroshi Nishimune Rachel O L Wong Joshua R Sanes

Neurites and other cell processes encounter localized deposits of signaling factors as they grow. The difficulty in generating patterned artificial substrates has hindered the analysis of these instructive factors in vitro. Here we report a simple method for presenting cultured cells with small spots of protein on an otherwise uniform substrate. We use a biolistic device called a gene gun to de...

Journal: :Cardiovascular research 1997
F C Tanner D P Carr G J Nabel E G Nabel

OBJECTIVE The introduction of recombinant genes into endothelial cells provides a method to study specific gene products and their effect on cell function. In addition, endothelial cells can be used for implantation into vessels or prosthetic vascular grafts. Because transfection efficiencies in human endothelial cells have been low, it is important to develop improved gene transfer techniques....

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