The aim of the present study was to examine effect quercetin on survival and primary culture ovine spermatogonial stem cells (SSCs). two-time enzymatic digestion process employed obtain SSCs from lamb testes. In next step, use filtration differential plating methods caused an increase in number cell suspension resulting mechanical digestions. Mitomycin-C-treated Sertoli were used prepare feeder...

This study investigated the effects of three proteases (trypsin, pepsin and chymotrypsin) on the hydrolysis efficiency of porcine placenta and the molecular weight (Mw) distributions of the placental hydrolysates. Because placenta was made up of insoluble collagen, the placenta was gelatinized by applying thermal treatment at 90 ℃ for 1 h and used as the sample. The placental hydrolyzing activi...

DNA methylation is an important epigenetic modification in many species that is critical for development, and implicated in ageing and many complex diseases, such as cancer. Many cost-effective genome-wide analyses of DNA modifications rely on restriction enzymes capable of digesting genomic DNA at defined sequence motifs. There are hundreds of restriction enzyme families but few are used to da...

At the ends of bacteriophage P2 DNA, the S’-terminated strands are 19 nucleotides longer than the 3’-terminated strands. The complete nucleotide sequence of the two cohesive ends of P2 DNA has been determined. This was accomplished by repair synthesis, using Escherichia coIi DNA polymerase and labeled deoxyribonucleoside triphosphates with or without a ribonucleoside triphosphate, followed by p...

MOTIVATION The bottom-up tandem mass spectrometry (MS/MS) is regularly used in proteomics nowadays for identifying proteins from a sequence database. De novo sequencing software is also available for sequencing novel peptides with relatively short sequence lengths. However, automated sequencing of novel proteins from MS/MS remains a challenging problem. RESULTS Very often, although the target...

Database searching is the technique of choice for shotgun proteomics, and to date much research effort has been spent on improving its effectiveness. However, database searching faces a serious challenge of efficiency, considering the large numbers of mass spectra and the ever fast increase in peptide databases resulting from genome translations, enzymatic digestions, and post-translational mod...

A simulation methodology for predicting the time-course of enzymatic digestions is described. The model is based solely on the enzyme’s sub-site architecture and concomitant binding energies. This allows sub-site binding energies to be used to predict the evolution of the relative amounts of different products during the digestion of arbitrary mixtures of oligomeric or polymeric substrates. The...

Methods We recruited 63 Italian children, adolescents and young adults with JIA (mean age 16.21 + 7.11 SD yrs, 51 female and 12 males, female/male ratio 4.25 from 1 Unit of Paediatric Rheumatology and 1 Unit of Rheumatology, Transition Clinic. After informed consent, during routine laboratory tests, their genomic DNA was extracted from peripheral blood leukocytes, to analyze VDR polymorphisms b...

We propose here a new strategy for the exhaustive mapping of phosphorylation sites in the Xenopus laevis Cdc25 phosphatase, which regulates cell cycle progression in eukaryotic cells. Two different MS analyses in a linear IT were used to identify the phosphorylated residues. First, a data-dependent neutral loss (DDNL) analysis triggered the fragmentation of peptides that show enhanced neutral l...

The work describes the accelerated enzymatic digestion of several proteins in various solvent systems under microwave irradiation. The tryptic fragments of the proteins were analyzed by matrix-assisted laser desorption/ionization mass spectrometry. Under the influence of rapid microwave heating, these enzymatic reactions can proceed in a solvent such as chloroform, which, under traditional dige...