for the evaluation of autoantibodies in pemphigus vulgaris (pv), an indirect elisa assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (m.w.) of 130-160 kda. the evaluation of pemphigus igg, igm and iga autoantibodies in 75 patients and 50 control sera indicated that this indirect elisa assay was a useful method for the detection of ...

systemic sclerosis is a generalized disorder of connective tissue clinically characterized by thickening and fibrosis of the skin and by distinctive forms of involvement of internal organs. one of the hallmarks of systemic sclerosis is the presence of serum autoantibodies against a variety of nuclear and cytoplasmic antigens. the primary purpose of this study was to identify the autoantibodies ...

multiple sclerosis (ms) is an autoimmune disease characterized by recurrent episodes of demyelination and axonal lesion mediated by cd4+ t cells with a proinflammatory t helper (th)1 and th17 phenotypes, macrophages, and soluble inflammatory mediators. the overactive  pro-inflammatory  th1  cells  and  clonal  expansion  of  b  cells  initiate  an inflammatory cascade with several cellular and ...

Three murine monoclonal anti-ssDNA autoantibodies have been produced by fusion of spleen cells from NZB/NZW F1 hybrid mice with Sp2/0-Ag 14 murine myeloma cells. Specificity studies characterizing monoclonal anti - ssDNA autoantibodies revealed binding patterns for nucleotides. Clone E-4-1 showed preferential binding with mono-di-and polyguanosine. Clones E-4-4 and E-11-1 specitically bound mon...

beta 2 glycoprotein i (β2gpi) is a single chain 50 kda highly glycosylated glycoprotein at an approximate concentration of 4 μm in cells. the abundance of this protein in plasma and its high state of preservation indicate the important role of this protein in mammalian. in addition, β2gpi has a particular structure in the fifth domain, and is categorized as the major antigen recognized by autoa...

the diabetes is an autoimmune disease, in which the role of autoantibodies is of a specific importance. the appearance of these autoantibodies can be the first symptom in the serum of type i diabetic patients, which may appear ten years before onset of the disease. the most important autoantibodies include glutamic acid decarboxylase autoantibodies (gad65-ab). this can be used as a good tool fo...

autoimmune hepatitis is a chronic hepatitis that occurs in children and adults of all ages. diagnosis is based upon characteristic serologic and histologic findings and the exclusion of other forms of chronic liver disease. guidelines issued by the aasld suggest the following diagnostic considerations: the diagnosis should be made in patients with compatible clinical signs, symptoms, and labora...

objective: to investigate possible immunological humoral correlates in newly diagnosed adult-onset generalized tonic-clonic epilepsy among iranian patients before and after sodium valproate treatment.   patients and methods: 72 adult patients with newly diagnosed idiopathic generalized tonic-clonic epilepsy were recruited. serum antinuclear antibodies (ana), anti-cardiolipin antibodies (acl), a...