Problem statement: The ability of a blast cyst to hatch, or escape, from the Zona Pellucida (ZP) that surrounds and protects the embryo during its first few days of development is one of many critical events that must occur for successful reproduction. Implantation of the embryo in the uterine lining is impossible unless hatching occurs. Hard or thick ZP will therefore impaire the implantation ...

BACKGROUND In MII oocytes showing difficult oolemma breakage, ICSI can cause an increase in the degeneration rate. This may be overcome by laser-assisted ICSI using a 5-10 micro m opening in the zona pellucida for injection. However, such a small opening might impair the hatching process, especially if assisted hatching is applied in addition. In order to prevent this, the present study used ro...

BACKGROUND Extended in vitro embryo culture and blastocyst transfer have emerged as essential components of the advanced reproductive technology armamentarium, permitting selection of more advanced embryos considered best suited for transfer. AIM OF STUDY The aim of this study was to compare between cleavage stage and blastocyst stage embryo transfer in patients undergoing intracytoplasmic sp...

OBJECTIVE To assess the effect of implementing different previtrification interventions on the postwarming DNA integrity of vitrified blastocysts. DESIGN Prospective in vitro study. SETTING Center for Reproductive Medicine laboratory in a tertiary hospital setting. ANIMALS A total of 70 expanded and 46 nonexpanded mouse blastocysts were used for the study. INTERVENTION(S) [1] Twenty-two...

STUDY QUESTION Does laser-assisted zona thinning of cleavage stage mouse embryos facilitate hatching in vitro? SUMMARY ANSWER No, unlike laser zona opening, zona thinning does not facilitate embryo hatching. WHAT IS KNOWN ALREADY Artificial opening of the zona pellucida facilitates hatching of mouse and human embryos. Laser-assisted zona thinning has also been used for the purpose of assist...

We exhibit an data graphic poster that emulates the style of historic hand-made visualizations of the 18th–19th century. Our visualization uses real data and employs style elements such as an emulation of ink lines, hatching and cross-hatching, appropriate typesetting, and unique style of computer-assisted facial drawings.

This study was designed to gain insight into the influence of spraying and cooling, during artificial incubation, on the embryo metabolic rate and hatching ability of Muscovy duck eggs. Three times a week 93 incubated eggs were sprayed and cooled for 0.5 h at room temperature. Daily embryo metabolic rate was measured in 30 eggs with a water vapor conductance ranging from 1.15 to 2.07 mg/day-kPa...

We exhibit an data graphic poster that emulates the style of historic hand-made visualizations of the 18th–19th century. Our visualization uses real data and employs style elements such as an emulation of ink lines, hatching and cross-hatching, appropriate typesetting, and unique style of computer-assisted facial drawings.