Antibodies to three isolates each of Armillaria mellea, A. ostoyae, A. tabescens, and Lentinula edodes were isolated from eggs of immunized laying hens. The reactivity of each antibody preparation with all isolates was examined using an enzyme-linked immunosorbent assay (ELISA). The cross-reactivity of the antibody preparations to a given Armillaria species varied considerably when tested again...

Three new sesquiterpene aryl esters and eight known compounds were isolated from the EtOH extract of the mycelium of Armillaria mellea. The structures of new compounds were established by analysis of their spectroscopic data. Some of the isolates showed cytotoxicity to a variety of cancer cell lines, including MCF-7, H460, HT-29, and CEM.

Phylogenetic and genetic relationships among 10 North American Armillaria species were analysed using sequence data from ribosomal DNA (rDNA), including intergenic spacer (IGS-1), internal transcribed spacers with associated 5.8S (ITS + 5.8S), and nuclear large subunit rDNA (nLSU), and amplified fragment length polymorphism (AFLP) markers. Based on rDNA sequence data, the nLSU region is less va...

Twenty-six isolates representing nine North American Armillaria species were investigated with flow cytometry and RFLP (restriction fragment length polymorphism) analyses to determine their nuclear DNA content and RFLP profile. Three putatively diploid isolates of A. ostoyae, A. gemina, A. calvescens, A. sinapina, A. mellea, A. gallica, A. nabsnona, and North American Biological Species (NABS) ...

Armillaria is a primary root rot pathogen of tea (Camellia sinensis) in Kenya. The main species presently described in this country are A. mellea and A. heimii. A survey covering fourteen districts of Kenya was carried out and forty-seven isolates of Armillaria collected. Cultural morphology, rhizomorph characteristics, somatic incompatibility and features of basidiomata were used to characteri...

An interaction study was performed with mycorrhizal plants of the grapevine rootstock Richter 110 (Vitis berlandieri Planch × Vitis rupestris Scheele) and the root pathogenic fungus Armillaria mellea (Vahl:Fr.) P. Kumm using an autotrophic in vitro culture system. Micropropagated plantlets were transferred to Petri plates with MSR medium lacking sugar and vitamins. Inocula of Glomus intraradice...

DNA sequences (~3 kb long) extending from the intergenic spacer 1 (IGS1) region to the 18S gene were obtained for isolates of Armillaria ostoyae, Armillaria calvescens, Armillaria gallica, and Armillaria sinapina. Additional investigation of 16 A. ostoyae, 11 Armillaria gemina, 21 A. calvescens, 18 A. gallica, and 15 A. sinapina isolates produced 117 sequences spanning the 3’ end of the IGS1 th...

Armillaria mellea is a traditional Chinese medicinal and edible mushroom. Many cultured products of A. mellea have been used to develop commercial medicines in recent years. The chemical composition and activities of the major bioactive chemical components-polysaccharides-may be different because of differences in the raw materials used. Four polysaccharides (SP, CMP, CFBP and CFMP) were obtain...