Five laboratory-adapted strains of Ureaplasma urealyticum showed comparable colony counts when incubated in eight gaseous environments including air, in different concentrations of carbon dioxide, and under anaerobiosis. For primary isolation on genital mycoplasma agar 95% N2-5% CO2 gave 100% correlation with growth in bromothymol blue broth, whereas certain strains failed to grow on agar incub...

1. Total lactate production (anaerobic energetic generation) during maximal activity was measured in eight species of amphibians. 2. Slow-moving animals, exemplified by Bufo boreas, produce small amounts of lactate and do not exhaust. In contrast, fast-moving, saltatory forms, such as Rana pipiens and Batrachoseps attenuatzls, have high levels of lactate generation and are unable to sustain max...

The question was asked whether the insensitivity to dihydrostreptomycin and streptomycin exhibited by facultatively anaerobic organisms growing strictly anaerobically could be due entirely to the lack of an anaerobic mechanism for the uptake of these antibiotics. A technique is described here which allows exposure of Escherichia coli B to dihydrostreptomycin, under conditions which are known to...

The anaerobic minimum inhibitory concentration of six aminoglycosides (amikacin, gentamicin, kanamycin, netilmicin, sisomicin, and tobramycin) averaged over 10-fold greater than the aerobic minimum inhibitory concentration for 50 clinical isolates of Staphylococcus aureus. Cultures from osteomyelitis and blood generally showed a somewhat greater increase in minimum inhibitory concentration due ...

The invasion of HEp-2 cells by Salmonella typhimurium was studied under various conditions. Anaerobiosis was shown to markedly affect the internalization of bacterial cells by HEp-2 cells. Anaerobically grown bacteria incubated with HEp-2 cells under anaerobic conditions markedly stimulated the rate of invasion. Anaerobiosis may therefore be a controlling factor in the invasion process. Cells o...