We describe an assay for 5-aminolevulinic acid dehydratase (EC4.2.1.24) activity. Radioactive [14Cjporphobilinogen, formed by action of this enzyme on [14CJo-aminolevulinic acid, is purified by passage through an ion-exchange chromatographic column before measurement with a liquid scintillation counter. The radioactive substance in the final solution was identified as solely [14C]porphobilinoge...

The activity of S-aminolevulinic acid (ALA) dehydratase. an enzyme involved in heme biosynthesis. has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study. the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase in A...

The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host -aminolevulinate dehydratase (ALAD), the second enzyme of the pathway, from the red cell cytoplasm by the intra erythrocytic malaria parasite has been demonstrated earlier in this laboratory. In this study, ALAD encoded by the Plasmodium falciparum genome (PfALAD) has been cloned, the protein overexpre...

The activity of S-aminolevulinic acid (ALA) dehydratase. an enzyme involved in heme biosynthesis. has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study. the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase in A...

The effects of lead, iron, copper, and zinc ions on 8-aminolevulinic acid dehydratase from red blood cell haemolysates in humans, both in the absence and presence of plasma proteins, have been investigated. 8-aminolevulinic acid dehydratase (ALAD) was not found to be a specific indicator of blood lead concentrations since it was also inhibited by copper and activated by zinc. Plasma protein pro...

Tishler and Winston recently described a screening test for delta-aminolevulinic acid in urine (1). The test is said to be useful for identification of an inherited deficiency of delta-aminolevulinic acid dehydratase (porphobilinogen synthase, EC 4.2.1.24). To complete the picture regarding this recently described inheritable biochemical lesion, we wish to note that the patients described by Do...

Aminolevulinic acid (ALA) is formed by the enzyme ALA synthase (hemA gene). Then ALA is converted to Porphobilinogen (PBG) by the ALA dehydratase (hemB gene). For the overproduction of ALA, we used an Escherichia coli BL21(DE3) containing a hemA gene from Bradyrhzobium japonicum, which was created in our previous work. The effects of pH on the ALA synthase and ALA dehydratase were investigated....