The aim of this study was to systematically compare the interaction of the long-acting beta2-adrenoceptor agonists formoterol and salmeterol with short-acting beta2-adrenoceptor agonists in contracted human bronchi. Human bronchi were obtained at thoracotomy from patients with lung cancer. Formoterol or salmeterol at concentrations inducing up to 92 and 94% of their maximal relaxant effect, res...

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 1. Physiology of Aqueous Humour and Intraocular Pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 2. Medical Treatment of Glaucoma . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ....

PURPOSE An investigation into whether alpha(2)-adrenoceptor agonists induce contractions in the porcine ciliary arteries and to characterize the functional receptor subtype mediating these responses. METHODS Isolated arteries from the intraocular part of the porcine ciliary artery were suspended in microvascular myographs for isometric tension recording. The segments were contracted with the ...

METHODS. Isolated arteries from the intraocular part of the porcine ciliary artery were suspended in microvascular myographs for isometric tension recording. The segments were contracted with the a2-adrenoceptor agonists brimonidine, apraclonidine, and oxymetazoline. To determine which subtypes of the a2-adrenoceptor mediate this contraction, antagonists subselective for the different a2-adreno...

McCormick C, Alexandre L, Thompson J, Mutungi G. Clenbuterol and formoterol decrease force production in isolated intact mouse skeletal muscle fiber bundles through a 2-adrenoceptorindependent mechanism. J Appl Physiol 109: 1716–1727, 2010; doi:10.1152/japplphysiol.00592.2010.—Although the acute actions of short-acting 2-adrenoceptor agonists on force production in isolated mammalian skeletal m...

Although the acute actions of short-acting β(2)-adrenoceptor agonists on force production in isolated mammalian skeletal muscle fibers have been the subject of a number of previous studies, those of long-acting β(2)-adrenoceptor agonists have never been investigated. Also, little is known about the cellular signal transduction events mediating their actions. Therefore, the primary aim of this s...

The alpha 2A-adrenergic receptor (adrenoceptor) was stably expressed in AtT20 mouse pituitary tumor cells; adrenoceptor agonists inhibited adenylyl cyclase, inhibited voltage-dependent calcium currents, and increased inwardly rectifying potassium currents. An aspartic acid residue (Asp79) highly conserved among guanine nucleotide-binding protein (G protein)-coupled receptors was mutated to aspa...

BACKGROUND β2-adrenoceptor agonists elicit bronchodilator responses by binding to β2-adrenoceptors on airway smooth muscle (ASM). In vivo, the time between drug administration and clinically relevant bronchodilation varies significantly depending on the agonist used. Our aim was to utilise a fluorescent cyclic AMP reporter probe to study the temporal profile of β2-adrenoceptor-mediated signalin...