histoid leprosy (hl) is a rare variant of lepromatous leprosy, occurring in long-standing cases, mostly in a background of acquired drug resistance. patients usually present with sudden onset multiple nodules and plaques, most often involving the skin and subcutaneous tissue of trunk and lower limbs. here we report an unusual case of de novo (without any history of prior anti-leprotic therapy) ...

Many of the computer science researchers now work for design automatic system for fast & accurate recognition of tuberculosis(TB), to speed up the treatment process of patient to save patient life. Any type of research is not possible without data. It is critical for computer researcher to prepare Ziehl-Neelsen stain sputum smear slide & to setup image acquisition technique on simple light micr...

Reduced acid-fast staining of leprosy bacilli occurs during the dewaxing of paraffin sections by xylene and alcohols; the older and more decrepit bacilli being especially affected. By the use of non-deparaffinized sections, the leprosy bacilli which could not be stained with the usual carbol fuchsin are strongly stained. Moreover, non-deparafinized sections can be used for the periodic acid-car...

Open-lung biopsy in a child with chronic pneumonia revealed branched gram-positive acid-fast organisms, later identified as Actinomyces israelii. Like Nocardia asteroides, A israelii can be acid fast by the commonly used Putt stain. The pneumonia was cured with 12 weeks of penicillin treatment.

According to WHO one third of the world population have tuberculosis. The present study was undertaken to compare the efficacy of flurochrome(FI) stain with conventional Z-N stain in the diagnosis of pulmonary & extrapulmonary tuberculosis. 388 cases of suspected pulmonary tuberculosis were included in the study. All samples were screen for Acid Fast Bacilli (AFB) by Z-N & Fluorescent staining ...

The Ziehl–Neelsen (ZN) stain, also known as the acidfast stain, has been reported to be helpful in detection and identification of Schistosoma eggs. In histological sections, Schistosoma mansoni egg shells appear as ZN positive and Schistosoma haematobium shells as ZN negative. The staining target of the responsible ZN component (carbolfuchsin) in the shell is unknown. Because carbolfuchsin is ...

The Ziehl–Neelsen (ZN) stain is important in identifying organisms that are acid fast, principally Mycobacterium tuberculosis. However, decolorisation with a weaker acid concentration (for example 1% hydrochloric acid), often used in ZN staining in histology, can result in a wider variety of organisms appearing acid fast and can be a cause of misidentification. To illustrate this point, a patie...

As infection with Cryptosporidium parvum becomes increasingly recognized as a cause of outbreaks of foal diarrhea, a rapid method of oocyst detection in equine feces that can be done easily in the practice setting is desirable. Protozoal identification techniques that have been described include the evaluation of stained fecal smears or preparations of floated feces, using both light and phase-...

dear editor,i read with interest article that published entitled *isolation and identification of bioactive compound producing rhodococcus spp. isolated from soil samples {ijmcm/5(1) (2015) 463-468}* [1]. some of the genus such as nocardia, gordonia, mycobacterium and rhodococcus are in actinomycete family and they are gram-positive and partially acid-fast. rhodococcus species usually stain gra...