The nature of the receptors for mucoid and nonmucoid Pseudomonas aeruginosa was investigated by using adherence to injured tracheal epithelium as a model. Bovine submaxillary mucin and crude rat tracheal mucin inhibited the adherence of both types of P. aeruginosa. Among the sugars present in these mucins only N-aceylneuraminic acid inhibited adherence. Inhibition of adherence probably involved...

Extracts and tissue culture supernatants of axenic forms of T. rangeli were assayed for the presence of sialidase and trans-sialidase activities. Using sialyl(alpha 2-3)lactose, sialyl(alpha 2-6)lactose, poly(alpha 2-8)N-acetylneuraminic acid, fetuin and 4-methylumbelliferyl-N-acetylneuraminic acid as sialic acid donors, and lactose as a sialic acid acceptor, no trans-sialidase activity was det...

The sialic acids (1) have in common a 9 carbon carboxylic acid, neuraminic acid (Fig. l), which may be regarded as a condensation product of a 3 carbon and a 6 carbon unit. Neuraminic acid itself is not found naturally, but several Nand O-substituted derivatives are widely distributed in nature (1). Chemical (2-4) and enzymic (5-7) degradations of N-acetylneuraminic acid have shown that this su...

N-Acetylneuraminic acid is produced by alkaline epimerization of N-acetylglucosamine to N-acetylmannosamine and then subsequent condensation with pyruvate catalyzed by free N-acetylneuraminic acid aldolase. The high-alkaline conditions of this process result in the degradation of reactants and products, while the purification of free enzymes to be used for the synthesis reaction is a costly pro...

Treatment of N-acetylneuraminic acid with excess base in the presence benzyl bromide gives a polyhydroxylated 1,4 lactone which after acetylation gave title compound 20% overall yield. The structure product was confirmed by single crystal X-ray diffraction analysis, as well FT-IR, NMR spectroscopic and HRMS analysis.

An enzyme, cytidine monophosphate sialic acid synthetase, was purified from hog submaxillary glands. This enzyme (or enzymes) catalyzes the synthesis of CMP-N-acetylneuraminic acid or CMP-N-glycolylneuraminic acid or both and inorganic pyrophosphate from CTP and the corresponding sialic acids. The preparation was inactive with other known sialic acids and analogues of these compounds, and was a...

The data presented in this article describe an effect of N-acetylneuraminic acid and/or quercetin on the inflammatory proteins expressions (TNF-α, ICAM-1, VCAM-1 and MOMA-2) and the N-acetylneuraminic acid (NANA) levels of apolipoprotein E-deficient mice that are given a high-fat diet. Protein expression was performed by immunohistochemical imaging and NANA was quantified by liquid chromatograp...

Adult male rats, under starving and normal conditions, were injected intravenously with N-acetyl[3H]mannosamine and after various time intervals the specific radioactivities of free N-acetylneuraminic acid (NeuAc) and CMP-N-acetylneuraminic acid were determined in the liver. The specific radioactivity of free NeuAc was high even within 20s after injection; the maximum was reached between 7 and ...