Here, we describe a fast, easy-to-use, and sensitive method to profile in-depth structural micro-heterogeneity, including intricate N-glycosylation profiles, of monoclonal antibodies at the native intact protein level by means of mass spectrometry using a recently introduced modified Orbitrap Exactive Plus mass spectrometer. We demonstrate the versatility of our method to probe structural micro...

by immunizing mice with killed whole bacterial cells of brucella abortus s (99), a panel of six hybridomas producing monoclonal antibodies (mab) specific for the surface antigens of this bacterium were produced. elisa was used to screen the hybridoma supernatants. immunoblots of the cell extract indicated that three mab were specific for s-lps (ba-1, ba-2, ba-3) and three others were reactive w...

background : production of monoclonal antibodies to leishmania antigens assists the identification and characterization of these organisms. objective: production of monoclonal antibodies against epitopes on the gp63. methods: two murine monoclonal antibodies to gp63 were produced and characterized. the reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

We studied the effects of growth hormone (GH), insulin-like growth factor I (IGF-I), IGF-II, and insulin on human immunoglobulin E (IgE) and IgG4 production. GH and IGF-I induced IgE and IgG4 production by normal donors' mononuclear cells (MNC) depleted of sIgE+ and sIgG4+ B cells without affecting IgM, IgG1, IgG2, IgG3, IgA1, or IgA2 production, whereas IGF-II and insulin failed to do so. GH-i...

Pemphigus vulgaris (PV) is characterized by IgG1 and IgG4 autoantibodies to desmoglein (Dsg) 3, causing suprabasal blistering of skin and mucous membranes. IgG4 is the dominant autoantibody subclass in PV and correlates with disease activity, whereas IgG1 can be associated with remittent disease. It is unknown if switching the same variable region between IgG4 and IgG1 directly impacts pathogen...

Patients treated with erythropoietin-based erythropoiesis-stimulating agents (ESAs) can develop a rare but life-threatening condition called antibody-mediated pure red cell aplasia (amPRCA). The antibody characteristics in a nephrology patient with amPRCA include high antibody concentrations with neutralizing activity and a mixed IgG subclass including anti-ESA IgG4 antibodies. In contrast, ant...

alpha-fetoprotein (afp), a serum glycoprotein belonging to the onco-developmental proteins group, serves as a marker both in cancer research and in studies concerning fetal development and fetal pathophysiology. monoclonal anti-afp antibodies are essential reagents in developing appropriate techniques for measurement of this protein. in this study, in order to produce anti-afp monoclonal antibo...

Dupilumab, a fully human IgG4 monoclonal antibody targeting the interleukin-4 receptor alpha (IL4Rα), substantially improves disease severity in patients with atopic dermatitis (AD). While recent daily practice studies have shown that dose reduction of dupilumab is possible controlled AD, associated biological effects are currently unknown. Therefore, we studied levels serum and on IL4Rα occupa...

METHODS. An extracapsular lens extraction (ECLE) was performed in 72 Sprague–Dawley rats. At the end of the procedure, 10 L TGF2 (TGF2–treated group), fetal calf serum (FCS)/phosphate-buffered saline (PBS; FCS/PBS-treated control group), a human monoclonal TGF2 antibody (anti–TGF2– treated group), or a null control IgG4 antibody (null antibody– treated control group) was injected into the capsu...