Epitope specificity and isotype influence mAb efficacy against Cryptococcus neoformans; however, the relative contribution of each attribute is poorly understood. To date, only mAbs that recognize two epitopes of capsular glucuronoxylomannan (GXM), defined by the IgG1 mAbs 2H1 and E1, consistently mediate protection against C. neoformans. The role of epitope specificity was further examined usi...

a Department of Forest Resource Management, 2424 Main Mall, University of British Columbia, Vancouver, British Columbia, V6T 1Z4, Canada b Department of Renewable Resources, Faculty of Agricultural, Life, and Environmental Sciences, University of Alberta, Edmonton, Alberta, T6G 2H1, Canada c Forest Management Branch, Forestry Division, Alberta Agriculture and Forestry, 9920-108 Street NW, Edmon...

The variable regions of the heavy and light chains of the protective murine monoclonal antibody (MAb) 2H1 (m2H1) were expressed with the human constant region genes for immunoglobulin G2 (IgG2) and kappa, respectively, to construct a chimeric antibody (ch2H1). ch2H1 retains the specificity of the parent MAb, exhibits biological activity, and lacks the toxicity of the parent murine IgG1 in chron...

J. S. Sperry,1* U. G. Hacke,y T. S. Feild,z Y. Sano,§ and E. H. Sikkema* *Department of Biology, University of Utah, 257S 1400E, Salt Lake City, Utah 84112, U.S.A.; yDepartment of Renewable Resources, University of Alberta, Edmonton, Alberta T6G 2H1, Canada; zDepartment of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, Tennessee 37996, U.S.A.; and §Laboratory of Woody Pla...