Designing potent silencing triggers is key to the successful application of RNA interference (RNAi) in mammals. Recent studies suggest that the assembly of RNAi effector complexes is coupled to Dicer cleavage. Here we examine whether transfection of optimized Dicer substrates results in an improved RNAi response. Dicer cleavage of chemically synthesized short hairpin RNAs (shRNAs) with 29-base-...

cocktail” drugs—protease inhibitors—were first approved, uninsured people with HIV disease waited up to 4-1/2 months longer, on average, than privately insured patients to begin life-sustaining medication, according to a new national study sponsored by the Agency for Health Care Policy and Research. The study also found that HIV patients covered by Medicaid waited 3 months longer than privately...

OBJECTIVE To evaluate our long-term experience with laparoscopic partial nephrectomy (LPN) and to review the literature. MATERIAL AND METHODS We performed a retrospective chart review, evaluating 227 consecutives laparoscopic partial nephrectomies performed between June 1995 and June 2010. Perioperative were recorded along with clinical a oncological outcomes. RESULTS Mean age was 56.4 year...

Alzheimer’s disease (AD) and many other neurodegenerative disorders belong to the family of protein misfolding diseases. These diseases are characterized by the deposition of insoluble protein aggregates containing an enriched β-sheet structure. To evaluate PET amyloid-imaging tracer [11C]BF-227 as an agent for in vivo detection of various kinds of misfolded protein, a [11C]BF-227 PET study was...

Neste artigo, discutiremos, a partir da anĂĄlise espacial como o projeto literĂĄrio de João Melo ĂŠ estruturado. Os contos, «Efeito Estufa» e «O Cortejo», trazem um tipo narrador que exemplifica esse modo ver cidade, tece informações respeito dos personagens espaços, colocando-se indivĂ­duo compreende as especificidades do narrado. Esse tem foco mostrar estratifi...

Este artigo analisa de forma breve o conto Conceição Evaristo, intitulado «Olhos d´ågua» (2014), sob prisma da memĂłria e identidade. MemĂłria identidade são indissociĂĄveis, sendo que a primeira representa capacidade conservar certas informações identidade, caracterĂ­sticas permitem se individualize as pessoas ou coisas. Assim, aglutina os processos permitindo cons...