Brinjal little leaf (BLL) is a widespread disease of phytoplasma etiology in India that induces severe economic losses. Surveys were conducted in eight brinjal-growing states of India during July 2014 to September 2015 and eighteen BLL samples showing little leaf, phyllody and witches' broom symptoms were collected for phytoplasma identification. Presence of phytoplasmas was confirmed in all th...

Fig (Ficus carica) trees exhibited symptoms of little leaf, mottling and deformation in Sanliurfa province, Turkey. Poylmerase chain reaction (PCR) through direct nested-PCR, phylogeny based on the sequence analysis revealed that infecting agent was closely related to ‘Candidatus Phytoplasma aurantifolia’ (16SrII-B subgroup). This is first report Ca. P. aurantifolia fig

Artemisia sieberi (Asteraceae) is an important perennial plant that common in the pastures, mountains and deserts of Iran. In 2019, A. showing little leaf, yellowing, internode shortening witches’-broom symptoms (Fig. 1) were observed around Abarkooh (Yazd province, Iran). The disease incidence was up to 5%. Total DNA extracted from 0.2 g fresh midrib tissue 10 diseased five symptomless plants ...

Peanut (Arachis hypogaea, Fabaceae), also known as groundnut, is an important oilseed and food legume crop cultivated in a wide range of climatic conditions across the globe. India second largest producer peanut world after China (Food Agriculture Organization United Nations, 2022). plants exhibiting phyllody, little leaf, excessive shoot proliferation, negative geotropism pegs with absence pod...

During 2021 surveys, greengage witches’-broom (GWB) and decline (GD) diseases were respectively observed in Fars Yazd provinces of Iran. Presence phytoplasma the symptomatic plants was confirmed by PCR amplifications 16S rRNA gene. Sequence analyses showed affiliation GWB GD phytoplasmas to‘Candidatus Phytoplasma phoenicium’ (16SrIX-B) and‘Ca. P. aurantifolia’ (16SrII-C) related strains, respec...

Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lu...

In September 2007, a peach tree (Prunus persica) displaying yellowing symptoms indicative of phytoplasma infection was sampled in Quba region of Azerbaijan. A phytoplasma was detected in the diseased peach tree by nested PCR amplification of its 16Sr DNA with universal primers for phytoplasmas. Phylogenetic analyses of the amplified 16S rDNA showed that the phytoplasma infecting peach tree corr...