BACKGROUND Trigonella foenum-graecum (L.) (Fabaceae, Fenugreek) is an important ingredient of Ayurvedic and other marketed herbal formulations. Fenugreek seeds are employed in many traditional systems as an antibacterial and antidiabetic agent, gastric stimulant and galactogogue. Trigonelline, a major phytoconstituent found in fenugreek seeds, shows estrogenic, anti-diabetic and anti-invasive a...

High-performance liquid chromatography (HPLC) methods employing ultraviolet (UV) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. Therefore, in the present study a simple, rapid and yet sensitive HPLC method with UV detection was developed and validated for quantitation of imipramine in human plasma samples. An efficient ...

In the presented study, the HPLC method was used for the determination of impurities (p-aminobenzoic acid and p-aminobenzoyl-L-glutamic acid) in single-component pharmaceutical products containing folic acid. The determination was performed using a spectrophotometric detector at lambda = 269 nm wavelength.

tetracyclines (tcs) are broad-spectrum antibiotics that are used widely in veterinary medicine. the present study was carried out to trace the residues of oxytetracycline (otc) and tetracycline (tc) in pasteurized milk that is marketed in tehran with the use of a high performance liquid chromatography (hplc). ninety milk samples were collected during five sequential days from the products of si...

Hollow graphitic carbon nitride (HCN) ZrO2/g-C3N4 hybrid composites (HCN–ZR) and the target catalyst Fe/ZrO2/g-C3N4 (HCN–FZR) were prepared successfully by a solvo-thermal method and evaluated for photo-degradation of organic pollutants, i.e. MO, and herbicides, metsulfuron methyl (MTSM). These materials were characterized by a variety of techniques, including Fourier transform infrared spectro...

Nicotinamide adenine dinucleotide (NAD(+)) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD(+)-consuming enzymes. The abundance of NAD (+), NAD(+) biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysi...

In vitro chlorophyll a degradation by horseradish peroxidase in the presence of the resorcinol was investigated in this paper, and the influence of pH and temperature was particularly studied. Chlorophyll a degradation was followed by UV–Vis and HPLC. Chlorophyll a was degraded when hydrogen peroxide was added into reaction mixture containing chlorophyll fraction, horseradish peroxidase, resorc...

nents of biomolecules, such as polysaccharides, nucleic acids, glycolipids and glycoproteins. In addition, numerous secondary metabolites in plants, such as terpenoids, steroids, and flavonoids, exist as glycosides, which conjugate with aldoses. Aldoses are optically active compounds, and confirmation of absolute configuration is required in natural product chemistry. Measurement of specific ro...

Methods were developed for the separation, detection, and quantification of tocopherols and phytosterols by high-performance liquid chromatography with an evaporative light-scattering detector. Four tocopherolsa, fJ, y and o-and four phytosterols-campesterol, fJ-sitosterol, brassicasterol, and stigmasterol-were analyzed in soybean, sunflower, low-erucic acid rapeseed (LEAR) and com oils. The us...

Soybean contains nutritional bioactive compounds, including carotenoids associated with human health benefits. Carotenoids are applicable in pharmaceuticals/nutreceuticals, cosmetic, and mainly food industries. However, an efficient accurate method for carotenoid chlorophyll detection quantification has not yet been developed validated soybean seeds. The need a rapid reliable analysis become in...