In 1947 we (1) described a microcolorimetric method, with a range of sensitivity from 10 to 60 y, for the determination of citric acid in small samples of blood and urine. This method was based on the conversion of citric acid to pentabromoacetone and on the subsequent reaction between pentabromoacetone and alcoholic sodium iodide with the development of a yellow color complex. Hydrazine sulfat...

In 1947 we (1) described a microcolorimetric method, with a range of sensitivity from 10 to 60 y, for the determination of citric acid in small samples of blood and urine. This method was based on the conversion of citric acid to pentabromoacetone and on the subsequent reaction between pentabromoacetone and alcoholic sodium iodide with the development of a yellow color complex. Hydrazine sulfat...

A new method is described for qualitative and quantitative determination of urinary homogentisiC acid. The method involves extraction of urine with ethyl acetate, thin-layer chromatography of the extract on Silica Gel G, elution of the homogentisiC acid into water, and color development with Folin’s phenol reagent. Absorbance is maximum at 750 nm and linear to a concentration of at least 5 mg o...

A 92-year-old African-American woman suffered from smoke inhalation during ahousefire.Thepatientwas transferred from a cross-town hospital to receive hyperbaric oxygen (HBO) therapy for carbon monoxide intoxication. Before arrival shewas sedated, intubated, and administered hydroxocobalamin (CyanoKit ; King Pharmaceuticals, Bristol, TN) 5 g intravenously for presumed cyanide poisoning. In the E...

The purpose of this study was to assess the influence of rehydration with a caffeinated beverage during nonexercise periods on hydration status throughout consecutive practices in the heat. Ten (7 women, 3 men) partially heat- acclimated athletes (age 24 +/-1y, body fat 19.2 +/- 2 %, weight 68.4 +/- 4.0 kg, height 170 +/- 3 cm) completed 3 successive days of 2-a-day practices (2 h/practice, 4 h...

Plasmodium vivax is the most prevalent cause of human malaria in the world and can lead to severe disease with high potential for relapse. Its genetic and geographic diversities make it challenging to control. P. vivax is understudied and to achieve control of malaria in endemic areas, a rapid, accurate, and simple diagnostic tool is necessary. In this pilot study, we found that a colorimetric ...

Measurement of serum ascorbate may be useful in long-term population studies because of the possible influence of ascorbate on numerous physiological factors. We describe an automated method for determining ascorbate in serum and urine by using the reduction of ferric iron by ascorbate and the formation of a color between the resulting ferrous iron and Ferrozine [3-(2-pyridyl)-5,6-bis(4-phenyls...

Congenital erythropoietic porphyria (CEP) is a rare cutaneous pophyria which presents with photosensitivity, complicated by presence of hemolytic anemia. There is no permanent cure and the treatment is supportive. A 3 day-old male baby was admitted with neonatal jaundice for phototherapy. The baby developed bullous lesions over both the feet within minutes of starting phototherapy(Fig.1). These...

Purple urine bag syndrome (PUBS) occurs in catheterized patients with bacterial urinary infections that produce sulphatase/ phosphatase. The presence of indigo and indirubin pigments, which are tryptophan metabolites, causes the purple coloring. After antibiotic therapy, colour reverts to normal. We present a case report unusual phenomenon occurring 60-year-old woman carcinoma cervix. She did n...