The most widely used ante-mortem diagnostic tests for tuberculosis in cattle are the tuberculin skin test and the interferon-gamma (IFN-γ) release assay, both of which measure cell-mediated immune responses to Mycobacterium bovis infection. However, limitations in the performance of these tests results in a failure to identify all infected animals. In attempting to increase the range of diagnos...

This study was conducted to determine the frequency of infected children cohabiting with sputum-positive and negative tuberculous adults for grounding authenticity Montoux test in diagnosing tuberculosis among these groups. It inferred from that a significant figure positive tuberculin skin not found be diseased those results were diagnosed based on chest X-ray, sputum examination, FNAC enlarge...

The same techniques may be used in the complement fixation test (CFT) for the serological diagnosis of bovine brucellosis, Johne's disease (paratuberculosis), dourine, equine piroplasmosis and Q fever (caused by Coxiella burnetii). The reproducibility of results is excellent, falling for the most part within the twofold range and never exceeding the fourfold range. Agreement with other laborato...

1. Rabbits infected with bovine tubercle bacilli develop hyper-sensitiveness to an intracutaneous injection of tuberculin. This sensitiveness appears from the 2nd to the 6th week after infection and increases rapidly thereafter. Tests, as a rule, show fluctuation in the intensity of the sensitization. Sensitization is followed by an interval of several weeks preceding death during which the ani...

Various aspects associated with anergy to tuberculin were investigated in a total of 75 tuberculous cattle. Forty-six (59%) had single lesions and 32 (41%) had multiple lesions. Forty-three (55.1%) had lesions in the lungs or associated lymph nodes and 23 (29.5%) showed multiple involvement of these tissues. Nine anergic cattle had generalised tuberculosis. Histological examination of lesions f...

The clinical usefulness of ex vivo interferon-gamma assays may largely depend on the assay format and epidemiological status of tuberculosis (TB) in the region studied. From July 2004 to June 2005 a prospective comparison study was undertaken at a tertiary referral hospital in South Korea. The results of tuberculin skin tests (TST) and the commercially available QuantiFERON-TB Gold (QFT-G) and ...

BACKGROUND Tuberculin testing remains the standard method for assessing infection due to tuberculosis in the UK. However, there are no data on the incidence of tuberculin positivity among the general adult population in the UK and consequently interpretation of Heaf test results is often difficult. METHODS During the investigation of an outbreak of tuberculosis in a Liverpool social club 198 ...

1. When toxic filtrates from cultures of B coli, B. typhosus, or meningococci are injected into the blood stream, peritoneal cavity, or subcutaneous tissue of tuberculous guinea pigs, the skin at the site of a tuberculin reaction becomes hemorrhagic. The extent of the hemorrhage is proportional to the severity of the tuberculin reaction demonstrable by tests with various dilutions of tuberculin...

A highly purified pneumococcal polysaccharide (Type II SSS) is a very efficient inducer of delayed hypersensitivity in random-bred guinea pigs. The cellular reactivity induced by this polysaccharide administered subcutaneously in complete Freund's adjuvant is of "tuberculin type"; it increases in intensity with time after the sensitizing injection, as judged by skin tests, the macrophage inhibi...

tuberculosis is an important opportunistic disease among hiv- infected persons worldwide; from march 1999 to feburery 2001 we had seventeen patients with hiv-infection and tuberculosis. here we are presenting the clinical manifestations, diagnosis, treatment and outcome of them. all of them were male, 11 patients had pulmonary and 5 patients had extrapulmonary (pleural effusion 1, hepatic granu...