Two viruses were isolated from cultured sole, Solea senegalensis, and wild blackspot sea bream, Pagellus bogaraveo, and preliminarily characterized as lymphocystis disease viruses (LCDVs). Viral isolates were characterized by morphological, biochemical and biophysical properties. In addition, the susceptibility of four fish cell lines was also tested. LCDV isolates developed cytopathic effects ...

To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and t...

Mammalian reovirus (MRV) causes respiratory and intestinal disease in mammals. Although MRV isolates have been reported to circulate several animals, there are no reports on Korean from wildlife. We investigated the biological molecular characteristics of based nucleotide sequence segment 1 gene. In total, 144 swabs wild animals were prepared for virus isolation. Based isolation with specific c...

We previously reported the isolation of the aneuploid MCH line of Chinese hamster cells from a normal line originally derived by Dr. George Yerganian from female adult fibroblast derivatives which formed a short-term carrier state with herpes simplex virus.1 We also reported the occurrence of chromosomal aberrations during the first postinfection division with this virus, and subsequently the p...

Evidence suggests that patients with long COVID can experience neuropsychiatric, neurologic, and cognitive symptoms. However, these clinical data are mostly associational studies complicated by confounding variables, thus the mechanisms responsible for persistent symptoms unknown. Here we establish an animal model of long-lasting effects on brain eliciting mild disease in K18-hACE2 mice. Male f...

The article presents the results of study toxic and virucidal action disinfectant ‘Biolaid’, which includes hydrogen peroxide, lactic acid, supralactic acid. research was conducted following ‘Methodical Approaches to Control Disinfectants for Veterinary Medicine’ (Kovalenko Nedosiekov, 2011). Toxicity ‘Biolaid’ characterized in SPEV BHK-21/C13 cell cultures (ATCC CCL-10). Determination activity...

Neutralizing antibodies (NA) inherently present in pooled plasma collected at commercial abattoirs may provide some protection against potential porcine circovirus type 2 (PCV2) infectivity of plasma. Moreover, these NA may also contribute to the biosafety of spray-dried porcine plasma (SDPP). The objective of the study was to characterize and quantify the PCV2 antibody neutralizing capacity in...

Neutralizing antibodies (NA) inherently present in pooled plasma collected at commercial abattoirs may provide some protection against potential porcine circovirus type 2 (PCV2) infectivity of plasma. Moreover, these NA may also contribute to the biosafety of spray-dried porcine plasma (SDPP). The objective of the study was to characterize and quantify the PCV2 antibody neutralizing capacity in...

Research has demonstrated that swine feed can be a fomite for viral transmission and additives reduce contamination. Therefore, the objective of this study was to evaluate two in contaminated with PEDV or PRRSV. Feed included: no treatment, 0.33% commercial formaldehyde-based product, 0.50% medium chain fatty acids (MCFA) blend. samples were inoculated PRRSV alone together at an inoculation con...