Site-specific and adaptable DNA binding domains are essential modules to develop genome engineering technologies for crop improvement. Transcription activator-like effectors (TALEs) proteins are used to provide a highly specific and adaptable DNA binding modules. TALE chimeric nucleases (TALENs) were used to generate site-specific double strand breaks (DSBs) in vitro and in yeast, Caenorhabditi...

Three-amino-acid-loop-extension (TALE) homeodomain proteins including Meis and Pbx families are generally recognized for their roles in growth and differentiation during vertebrate embryogenesis and tumorigenesis. Whereas genetic studies indicate that Pbx1 regulates the development and function of insulin-producing pancreatic β-cells, the role of Meis family members in β-cells is still unknown....

The ability to perturb individual genes in genome-wide experiments has been instrumental in unraveling cellular and disease properties. Here we introduce, describe the assembly, and demonstrate the use of comprehensive and versatile transcription activator-like effector (TALE) libraries. As a proof of principle, we built an 11-mer library that covers all possible combinations of the nucleotides...

An acute infectious epidemic almost eliminated the St Kilda community in 1727. An epic tale of survival in adversity followed. Contemporary records reveal atypical features, suggesting a speculative alternative.

This paper describes the implemented first prototype of a domainoriented, conversational edutainment system which allows users to interact via speech and 2D gesture input with life-like animated fairy-tale author Hans Christian Andersen.

This paper recounts the tale of a most vicious dragon that ate thousands of people every day, and of the actions that the king, the people, and an assembly of dragonologists took with respect thereof.

A few readers of my last two columns may have suspected that something was afoot. Why, they might ask, does he suddenly produce not one, but two confessions? After shamefacedly exposing his tendency to consider book theft as a way of acquiring knowledge and trying to reverse the hint of plagiarism in his works by suggesting that it was the other way around, we might expect anything. Well, the e...

Bioconjugate Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Cleavage of DNA by electrochemically activated manganese(III) and iron(III) complexes of meso-tetrakis(N-methyl-4-pyridiniumyl)porphine Marisol Rodriguez, Thomas Kodadek, Maritza Torres, and Allen J. Bard Bioconjugate Chem., 1990, 1 (2), 123-131 • DOI: 10.1021/bc00002a006 Downl...

Using chosen examples, the author analyses the meaning of the changes made by Vincent (Wincenty) Kadłubek also known as Master Vincentius, to the tales found in the chronicle of Gallus Anonymus. Nothing much can be said of the literary or oral sources which may have served as a basis for Vincentius’s reworking of these stories. A detailed comparison of the corresponding fragments in both chroni...