Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...

High titer and purity levels are key requirements for virus-based cancer gene therapy biopharmaceuticals. However, the task of removing enough host-cell DNA from therapeutic viruses in order to comply with FDA’s 10 ng/dose limit is particularly challenging. In a previous study, we demonstrated advantages using laterally-fed membrane chromatography (LFMC) adenovirus purification. Although this a...

The growing interest in chemical evaluation of thyroid function by use of the plasma level of protein-bound iodine (PI) is attested by the increasing numbers of reports in which this criterion is employed, both chemically and experimentally. However, the specific techniques available for such a determination do not yet seem sufficiently reliable. The purpose of this communication is to describe...

The effects of rapeseed and soybean expeller on digestion of sodium, potassium, calcium, magnesium, phosphorus and sulphur in dairy cows were investigated in a study conducted as an incomplete Latin square. The experimental diets consisted of five concentrates fed at a rate of 9 kg d-1: a mixture of barley and oats (control), which was replaced either with rapeseed or soybean expeller both at t...

The fermentation of the neutral detergent-soluble (NDS) fraction of two legumes (clover, alfalfa) and two grasses (timothy, guinea grass) was measured using a curve subtraction technique with in vitro gas production data from the whole forage and the isolated neutral detergent-extracted fiber. The NDF disappearance and VFA production also were measured. There were no significant differences bet...

The growing interest in chemical evaluation of thyroid function by use of the plasma level of protein-bound iodine (PI) is attested by the increasing numbers of reports in which this criterion is employed, both chemically and experimentally. However, the specific techniques available for such a determination do not yet seem sufficiently reliable. The purpose of this communication is to describe...

DNA isolation from hair shafts can involve a number of steps, each of which adds time to the procedure and increases the risk of contamination. A simple alkaline digestion procedure that directly dissolves hairs was developed and compared with a widely used glass grinding/organic extraction method, using samples collected from 30 volunteers with varying population ancestries, hair colors, and h...

Murine and human esophageal myofibroblasts are generated via enzymatic digestion. Neonate (8-12 day old) murine esophagus is harvested, minced, washed, and subjected to enzymatic digestion with collagenase and dispase for 25 min. Human esophageal resection specimens are stripped of muscularis propria and adventitia and the remaining mucosa is minced, and subjected to enzymatic digestion with co...

The simplified acid peroxydisulphate digestion of soil water extract was evaluated for determination of total dis solved phosphorus by molybdenum-blue colorimetry in comparison with direct P-detection in water extract by the ICP-AES technique. The research was conducted on 79 agricultural soils with different agrochemical characteristics. The results of the colorimetric P detection in water ext...

recently, particular attention has been paid to the stepped spillways due to the increasing effect of energy dissipation and the reduction of cavitations risks with the development of roller compacted concrete (rcc) technique. flow regimes on the spillways divide into three groups, namely skimming, jet and transition flow. compared to the numerical methods, the majority of performed studies in ...