Babesiosis is caused by intraerythrocytic protozoan parasites transmitted by ticks and affects a wide range of domestic and wild animals and occasionally humans. The current study aimed to investigate the effect of B. divergens infected erythrocytes on spleen histopathology, cell cycle alteration, and the presence of oxidative stress. Mongolian gerbils were challenged with 5 × 10(6)  Babesia di...

A/J anti-p-azobenzenearsonate (ABA) antibodies bearing cross-reactive idiotypic (CRI) determinants, when coupled to spleen cells and then injected intravenously into naive animals, stimulate suppressor T cell (Ts) responses. Moreover, previous studies have demonstrated that the ability of such idiotype-coupled spleen cells to induce immune unresponsiveness to subsequent immunization with ABA-co...

A cytostasis assay has been used to study the natural immunity of mice to murine mammary tumor virus (MTV). Spleen cells from adults of all strains tested were found to be cytostatic to a variety of MTV-positive mammary tumor cell lines. Newborn spleen cells were unreactive in the same cytostasis assay. The degree of reactivity to the target cells was greater in spleen cell preparations from lo...

a hybridoma clone designated 6g5 has been selected by fusion of mouse myeloma cell line ag. 8653 with spleen cells from mice immunized with human peripheral blood mononuclear cells (pbmc). the antibody produced by this clone was found to be strongly reactive with four human b-cell lines in the conventional immunological assays. despite the fact that expression of most b cell-associated markers ...

Hematopoietic cell lineages are best described in terms of distinct progenitors with limited differentiative capacity. To distinguish cell lineages, it is necessary to define progenitors and induce their differentiation in vitro. We previously reported in vitro development of immature dendritic-like cells (DCs) in long-term cultures (LTCs) of murine spleen, and in cocultures of spleen or bone m...

The fetal mouse omentum has been shown to be a source of precursors that exclusively reconstitutes Lyl + B cells and the closely related Lylsister population, but not conventional B cells or T cells. We have extended these studies to compare B cell development in the human fetal omentum, liver, and spleen, and to demonstrate that the pro/pre-B cell compartment (CD24 +, sIgM-) is detected in the...

The fetal mouse omentum has been shown to be a source of precursors that exclusively reconstitutes Ly1+ B cells and the closely related Ly1- sister population, but not conventional B cells or T cells. We have extended these studies to compare B cell development in the human fetal omentum, liver, and spleen, and to demonstrate that the pro/pre-B cell compartment (CD24+, sIgM-) is detected in the...

The spleen is one of the most frequently affected organs in sickle cell anemia (SCA). This study aims to characterize the spleen in sickle cell anemia patients using contrast enhanced computerized tomography scanning (CECT). 67 patients with SCA from different Saudi Arabian areas were enrolled; ages are ranged from 10 months to 28 years old. The spleen was assessed with CT for abdominal pain an...

PWM-activated spleen cell-conditioned medium (SCCM) and a variety of purified hematopoietic growth factors were tested for their ability to stimulate chemotaxis of mouse connective tissue mast cells (CTMC). Of the agents tested, only IL-3 and SCCM promoted mast cell chemotaxis. Neither IL-2, IL-4, GM-CSF, nor endotoxin had any significant mast cell chemotactic activity. Neutralizing antibodies ...