BACKGROUND: <span style="color: #221e1f; font-fami...

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...

Two trials were conducted to evaluate the influence of malic acid supplementation on ruminal fermentation. In Trial 1, six Holstein steers (300 kg) with ruminal cannulas were used in a crossover design experiment to study the influence of malic acid (MA) on ruminal metabolism during glucose-induced lactic acidosis. Treatments consisted of a 77% steam-flaked barley-based finishing diet supplemen...

Physically effective fiber is needed by dairy cattle to prevent ruminal acidosis. This study aimed to examine the effects of different sources of physically effective fiber on the populations of fibrolytic bacteria and methanogens. Five ruminally cannulated Holstein cows were each fed five diets differing in physically effective fiber sources over 15 weeks (21 days/period) in a Latin Square des...

Six adult castrated male red deer (Cervus elaphus), fitted with rumen cannulas, were offered chaffed lucerne hay ad lib. during winter and summer, with voluntary food intake (VFI) being respectively 59 and 89 g dry matter intake (DMI)/kg bodyweight 0.75 per d. The same animals were also offered the same feed during summer, with intake restricted to that of winter VFI. The apparent digestibility...

All mechanistic rumen models cover the main drivers of variation in rumen function, which are feed intake, the differences between feedstuffs and feeds in their intrinsic rumen degradation characteristics, and fractional outflow rate of fluid and particulate matter. Dynamic modeling approaches are best suited to the prediction of more nuanced responses in rumen metabolism, and represent the dyn...

I . The deamination, ureolytic and proteolytic activities and rates of cellulolysis and gas production in large intestinal contents have been compared with those activities and rates in rumen contents of sheep. 2. Large intestinal contents produced ammonia. The rate of ammonia production was greater in caecal contents than in faeces. Incubation of caecal contents under toluene resulted in decre...

objective(s)cardiovascular disease causes a major clinical problem in patients with end stage renal disease. since metabolic acidosis is very common in patients with end stage renal disease, we aimed to investigate the effect of experimentally-induced metabolic acidosis on serum lipid profile and aortic fatty streak (fs) formation in normal and high-cholesterol fed rabbits.materials and methods...

Dissolved hydrogen (dH) influences the pathways of VFA production and is a precursor of methane formation in the rumen. Measurements of dH in rumen fluid taken at the same time as measuring other rumen fermentation end products would improve our quantitative understanding of the role of dH as a controller of rumen fermentation. Sample collections though a rumen cannula and using oral stomach tu...