Two new dirhodium(II) complexes possessing the intercalating dppz ligand (dppz = dipyrido[3,2-a:2',3'-c]phenazine), cis-[Rh(2)(mu-O(2)CCH(3))(2)(dppz)(eta(1)-O(2)CCH(3))(CH(3)OH)](+) (1) and cis-[Rh(2)(mu-O(2)CCH(3))(2)(dppz)(2)](2+) (2), were synthesized and characterized as potential agents for photochemotherapy. Various techniques show that 1 binds to DNA through intercalation, although some...

A recombinant plasmid, pIP1607, containing the thermonuclease gene (nuc) of S.intermedius was detected in a pUC18 library, constructed by cloning cellular DNA of S.intermedius strain LRA076 partially digested with So«3A in the BamHl site. E.coli strain TGI was used as a recipient for DNA transformation. The screening method consisted in replica-plating the ampicillinresistant colonies on DNA-to...

Pseudomonas pseudoalcaligenes JS45 grows on nitrobenzene by a partially reductive pathway in which the intermediate hydroxylaminobenzene is enzymatically rearranged to 2-aminophenol by hydroxylaminobenzene mutase (HAB mutase). The properties of the enzyme, the reaction mechanism, and the evolutionary origin of the gene(s) encoding the enzyme are unknown. In this study, two open reading frames (...

removed. air dried. incubated in 7 X 1 0 I ' M-l"l-~abclkd cyanopindolol (CY P). washed, rapidly dried and subjected to autoradiography t o detect the presence of /3-adrenergic receptors [ 8 I. Autoradiography of polyester filter replicas after fusion ot COS cells with spheroplasts containing pPAR revealed punctate areas with high density "'I-CYP binding, consistent with induction o f /3-adrcne...

One drawback when using non-radioactive labelling has been that there is no easy test for the degree of incorporation of nucleotide analogues. Here we show that biotinylated DNA-fragments have a lower mobility than non-biotinylated fragments during agarose gel electrophoresis, and that this gives a good indication of the degree of end-labelling of DNA-fragments. This feature can be exemplified ...

In a living cell, a multiply damaged site in DNA is thought to be processed by several different pathways simultaneously or sequentially. Under this situation, the cellular response to the lesion cluster might depend on the order of repair processes because the configuration of the lesions will be modified by the reaction of initial repair protein, affecting the DNA-binding or lesion-excision a...

Polymerase chain reaction (PCR) methodology (1) has become a routine method for selectively amplifying segments of DNA from a wide variety of sources. Amplification of specific sequences is dependent upon an exact match between the template DNA and the oligonucleotide primers. Mismatches at the 3' terminus lead to greatly reduced amplification, with no detectable product when amplified under th...

A number of RecA-like proteins have been found in eukaryotic organisms. We demonstrate that the prokaryotic recombination protein RecA itself is capable of interacting with genomic homologous DNA in somatic plant cells. Resistance to the DNA crosslinking agent mitomycin C requires homologous recombination as well as excision repair activity. Tobacco protoplasts expressing a nucleus-targeted Rec...

The isolation of genes from a given genomic region can be a rate-limiting step in the discovery of disease genes. We describe an approach to the isolation of cDNAs that have sequences in common with large genomic clones such as bacterial artificial chromosomes. We applied this method to loci both amplified and deleted in cancer, illustrating its usage in the identification of both oncogenes and...