Neuropeptide Y (NPY) from the brain of an amphibian from the order Gymnophiona (the caecilian, Typhlonectes natans) was characterized. We cloned a 790 base pair cDNA encoding the caecilian NPY precursor. The open reading frame consisted of 291 bases, indicating an NPY precursor of 97 amino acids. Both deduced and isolated NPY primary structures were Tyr-Pro-Ser-Lys-Pro-Asp-AsnPro-Gly-Glu-Asp-Al...

ABRAMS, ROBERT, AND H. T. HAMMEL. Cyclic variations in hypothalamic temperature in unanesthetised rats. Am. J. Physiol. 208(4): 698-702. 1965. -Male and female rats, equipped with stainless steel re-entrant tubes implanted in the brain adjacent to one preoptic area, were placed in cages with accessible activity wheels. Brain temperatures were recorded continuously for periods up to 48 hr by mea...