DNA-PK/Gene expression/Hypoxia/Reoxygenation. In order to elucidate the mechanism of concerted expression of various proteins related to DNA-PK activity, we examined the relationship of the expression of these proteins with that of a transcription factor Sp1. We also explored whether the expression of genes related to DNA-PK activity and Sp1 changed after hypoxia and reoxygenation. RT-PCR was e...

Thioredoxin constitutes the prototype of the thiol-disulfide oxidoreductase family. These enzymes contain an active-site disulfide bridge with the consensus sequence Cys-Xaa-Xaa-Cys. The more N-terminal active-site cysteine is generally a strong nucleophile with an abnormal low pK(a) value. In contrast, the more C-terminal cysteine is buried and only little is known about its effective pK(a) du...

Using 4.5 fb−1 of e+e− annihilation data samples collected at the center-of-mass energies ranging from 4.600 GeV to 4.699 with BESIII detector BEPCII collider, a first study semileptonic decays Λc+→pK−e+νe, Λc+→Λ(1520)e+νe and Λc+→Λ(1405)e+νe is performed. The Λc+→pK−e+νe decay observed significance 8.2σ branching fraction measured be B(Λc+→pK−e+νe)=(0.88±0.17stat±0.07syst)×10−3. We also report...

Plasma kallikrein (PK) is a serine protease generated from plasma prekallikrein, an abundant circulating zymogen expressed by the Klkb1 gene. The physiological actions of PK have been primarily attributed to its production of bradykinin and activation of coagulation factor XII, which promotes inflammation and the intrinsic coagulation pathway. Recent genetic, molecular, and pharmacological stud...

For any metric spaces X and Y we denote by C(X,Y ) the class of all continuous functions from X into Y . Let N and Z denote the sets of natural numbers and integers respectively. Denote by S the set of all real sequences p = (pk)k∈N. For p = (pk)k∈N ∈ S, p̄ = (p̄k)k∈N ∈ S we write |p| = (|pk|)k∈N and p ≤ p̄ if pk ≤ p̄k for k ∈ N. For p = (p (m) k )k∈N ∈ S , m ∈ N and p = (pk)k∈N ∈ S we put lim m→∞ ...

The DNA-dependent protein kinase (DNA-PK) phosphorylates a number of transcription factors. Here, we show that the DNA-PK modifies c-Jun in vitro and that serine residue 249 (Ser-249) is required for phosphorylation to occur. This residue corresponds to one of three sites of c-Jun that are phosphorylated in vivo and which negatively regulate c-Jun DNA binding in vitro. However, we find that pho...

A point mutation (1 277 CGG to CAG) was identified in the R-type pyruvate kinase (PK) cDNA of a PK variant, PK Sapporo, associated with hereditary non-spherocytic hemolytic anemia. The mutation causes a single amino acid substitution from Arg to Gln at the 426th amino acid residue of human R-type PK; consequently, the hydrophobicity around the mutated site is drastically decreased. The amino ac...

• Gal and Gharamani (2015) reinterpreted dropout regularisation as approximate inference in BNNs •Dropout probabilities pl are variational parameters of the approximate posterior qθ(ω) = ∏ k qMk,pk(Wk), where Wk = Mk · diag (zk) and zkl iid ∼Bernoulli(1− pk) • Concrete distribution (Maddison et al., Jang et al.) relaxes Categorical distribution to obtain gradients wrt the probability vector – E...

To further investigate the erythroid nature of the two human erythroleukemia cell lines, K562 and HEL-60, and to define the ontogeny of pyruvate kinase (PK) isozymes (R, M2) in developing human erythroid cells, we have studied the isozymic alterations, if any, during differentiation of these cell lines in vitro and normoblasts isolated from fetal liver in vivo. PK activity of erythroleukemic ce...

By using the potentiometric titration method, we have determined the pK(a) values of the two terminal lysine groups in six alanine-based peptides differing in the length of the alanine chain: Ac-Lys-Lys-NH(2) (KK), Ac-Lys-Ala-Lys-NH(2) (KAK), Ac-Lys-Ala-Ala-Lys-NH(2) (KAK2), Ac-Lys-Ala-Ala-Ala-Lys-NH(2) (KAK3), Ac-Lys-Ala-Ala-Ala-Ala-Lys-NH(2) (KAK4), and Ac-Lys-Ala-Ala-Ala-Ala-Ala-Lys-NH(2) (K...