Abstract Introduction Early vaccination of cattle with an inactivated commercial bacterial vaccine against bovine respiratory disease has been reported to increase antibody production and can alleviate the disease. However, its dosage little investigated in young Holstein calves. This study addresses need establish guide values for these animals. Material Methods Healthy calves received Histoph...

In this study we developed a new method for the partial purification of Pasteurella haemolytica leukotoxin by size-exclusion high-performance liquid chromatography. The partially purified leukotoxin had a molecular weight of 104,000, as estimated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and reacted on an immunoblot with an antileukotoxin monoclonal antibody. As expect...

Solunum yolu enfeksiyonları çiftlik hayvanlarında ekonomik kayıplara, verim düşüklüğüne, ölümlere neden olan önemli hastalıklardandır. Bu çalışma ile Marmara bölgesindeki pnömonili sığır, koyun ve keçi akciğerlerinde görülen bakteriyel etkenlerin saptanması, bu ajanların yaş, tür, mevsim illere göre dağılımlarının istatistiksel olarak değerlendirilerek, güncel epidemiyolojik verilerin oluşturul...

Wild and domestic animal populations are known to be sources and reservoirs of emerging diseases. There is also a growing recognition that horizontal genetic transfer (HGT) plays an important role in bacterial pathogenesis. We used molecular phylogenetic methods to assess diversity and cross-transmission rates of Pasteurellaceae bacteria in populations of bighorn sheep, Dall's sheep, domestic s...

A temperature-sensitive (TS) plasmid was generated from the endogenous streptomycin resistance plasmid of Mannheimia hemolytica and used to engineer in-frame aroA deletion mutants of Mannheimia hemolytica, Pasteurella multocida, and Haemophilus somnus. TS replacement plasmids carrying in-frame aroA deletions were constructed for each target species and introduced into host cells by electroporat...