There is an urgent need to conserve and improve the quality of agricultural soils in coming decades. Decision tools capable providing reliable information about soil are needed, index (SQI) one most used. Principal component analysis (PCA) common methodology calculate it, however some cases fails differentiate properly. Therefore, aim this work assess a SQI through different as network (NTA) co...

Abstract This study investigates the influence of sea surface temperature (SST) in northern tropical Atlantic (NTA) on Indo–western Pacific summer climate by analyzing record-high NTA SSTs 2010. In that time, a decaying El Niño and developing La Niña were accompanied widespread anomalous conditions Pacific. These are typical summers follow events often explained as being due to Indian Ocean war...

The surface of a germanium internal reflectance element (IRE) was modified to bind 6X-histidine (his)-tagged biomolecules. The step-by-step surface modification was monitored via single-pass attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FT-IR). Initially an adlayer of 7-octenyltrimethoxysilane (7-OTMS) was formed on the Ge crystal through the surface hydroxyl groups,...

Networks of timed automata (NTA) are widely used to model distributed real-time systems. Quite often in the literature, the automata are allowed to share clocks, i.e. transitions of one automaton may be guarded by conditions on the value of clocks reset by another automaton. This is a problem when one considers implementing such model in a distributed architecture, since reading clocks a priori...

Particle tracking analysis (PTA) is an emerging technique suitable for size analysis of particles with external dimensions in the nano- and sub-micrometre scale range. Only limited attempts have so far been made to investigate and quantify the performance of the PTA method for particle size analysis. This article presents the results of a validation study during which selected colloidal silica ...

Introduction: Nanoparticle tracking analysis (NTA) enables measurement of extracellular vesicles (EVs) but lacks the ability to distinct between EVs and lipoproteins which are abundantly present in blood plasma. Limitations in ultracentrifugation and size exclusion chromatography applied for EV isolation may result in inadequate EV purification and preservation. In this proof of concept study, ...

Objective(s): Brucella spp. are facultative intracellular pathogens which can stay alive and multiply in professional and nonprofessional phagocytes. Immunity against Brucella melitensis involves antigen-specific CD4+ and CD8+ T-cells activation and humoral immune responses. Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antig...

Objective(s): Enterotoxigenic Escherichia coli (ETEC) is known as the most common bacterial causes of diarrheal diseases related to morbidity and mortality. Heat-labile enterotoxin (LT) is a part of major virulence factors in ETEC pathogenesis. Antigen entrapment into nanoparticles (NPs) can protect them and enhance their immunogenicity.Materials and Methods: In the present study, recombinant L...

In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...

INTRODUCTION Identifying ideal non typeable Haemophilus influenzae (NTHi) vaccine candidates has not been easy due to extensive sequence and antigenic variation among gene products interacting with the immune system. Protein D (PD) is a highly conserved 42 kDa surface lipoprotein available in all H. influenzae, including NTHi. METHODOLOGY In this study, the gene encoding PD was cloned from H....