We report on the immobilization of an OPAA enzyme on luminescent porous silicon devices, and on the utilization of this new platform to hydrolyze p-nitrophenyl-soman.

A beta-mannosidase (beta-D-mannoside mannohydrolase, EC 3.2.1.25) was purified to apparent homogeneity from the culture filtrate of the fungus, Aspergillus niger. The enzyme had an estimated molecular weight of about 120,000 and was a glycoprotein. Radioactive enzyme was prepared by growing the fungus in [14C]fructose, and this enzyme was used for the preparation of 14C-glycopeptides. The glyco...

A purified commercial preparation of Escherichia coli alkaline phosphatase (EC 3.1.3.1) has been shown to dephosphorylate several phosphoproteins including bovine heart glycogen synthase D, mixed phosphohistones, and rabbit skeletal muscle phosphorylase kinase but not rabbit skeletal muscle glycogen phosphorylase. Alkaline phosphatase completely removed phosphate groups previously added during ...

We have previously shown that cross-reactive sensitivity (CS) responses induced by 4-hydroxy-3-nitrophenyl acetyl-O-succinimide (NP-O-Su) and elicited by its 5-iodo analogue, 4-hydroxy-5-iodo-3-nitrophenyl acetyl-O-succinimide were observed in strains of mice possessing the Igh-1b allotype, but not in strains bearing allotypes Igh-1c or Igh-1j. These CS responses are mediated by T cells and can...

To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with Sc- and Rc-(Rp,Sp)-1,2-dialkylcarbamoylglycero-3-O-p-nitrophenyl alkylphosphonates of different alkyl chain lengths. P. cepacia lipase was most rapidly inactivated by Rc-(Rp,Sp)-1,2-dioctylcarbamoylglycero-3-O-p-nitrophenyl octylphosphonate (Rc-trioctyl) with an inactivation half-time of ...

We report herein a whole cell-based amperometric biosensor for highly selective, highly sensitive, direct, single-step, rapid, and cost-effective determination of organophosphate pesticides with a p-nitrophenyl substituent. The biosensor was comprised of a p-nitrophenol degrader, Pseudomonas putida JS444, genetically engineered to express organophosphorus hydrolase (OPH) on the cell surface imm...