PURPOSE Our goal was to improve and standardize the procedure for subretinal injection of mouse eyes. Also, we wished to optimize conditions for electroporation of retinal pigment epithelium (RPE) cells in the mouse eye with naked plasmids. METHODS Mouse eyes were injected subretinally with reporter plasmid DNA, nanobeads, or buffer. A blunt needle was introduced across the cornea, through th...

Measuring extracellular dopamine in the brain of living animals by means of microdialysis and/or voltammetry is a route towards understanding both normal brain function and pathology. Previous reports, however, suggest that the tissue response to implantation of devices may affect the outcome of the measurements. To address the source of the tissue response and its impact on striatal dopamine s...

The interest in patterning surfaces with microand nanometer resolutions has been growing fast in recentyears, illustrating the tremendous potential of patterning techniques for both fundamental investigationsand technological applications in photonics, molecular electronics, and biosensors. Among the variouspatterning methods, liquid dispensing techniques relying on the use of m...

A classical microscope resolves features of around half of the wavelength of illumination λ . [ 1 ] Breaking this limit is possible in near-fi eld scanning optical microscopy using a local evanescent illumination spot, [ 2 ] in integral imaging three-dimensional microscopy, [ 3 ] or by applying a material of negative refractive index, referred to as a ‘superlens’. [ 4–6 ] A far-fi eld superlens...

2010 WILEY-VCH Verlag Gmb Magnetic beads with functionalized surfaces are widely used as molecule carriers or labels for single molecule studies, cell manipulation, and biomagnetic sensing. For this reason manipulation at the nanoscale of surface functionalized magnetic beads in suspension is of paramount importance in biotechnology, nanochemistry, and nanomedicine as it leads to a precise cont...

Systems biology is the study of an organism, viewed as an integrated and interacting network of genes, proteins and biochemical reactions which give rise to life. Instead of analyzing individual components or aspects of the organism, systems biologists focus on all the components and the interactions among them, all as part of one system using computer-based or special algorithms-based analysis...

Microarrays of biomolecules and cells have played important roles in genomics, diagnostics, and drug screening. Live-cell capture on micropatterned surfaces is particularly valuable for diagnostic or biosensing applications, as cell arrays are amenable to rapid characterization and sorting. For example, antibody-coated micropost arrays have been used to detect and isolate rare circulating tumor...

Lambda phages have considerable potential as gene delivery vehicles due to their genetic tractability, low cost, safety and physical characteristics in comparison to other nanocarriers and gene porters. Little is known concerning lambda phage-mediated gene transfer and expression in mammalian hosts. We therefore performed experiments to evaluate lambda-ZAP bacteriophage-mediated gene transfer a...