OBJECTIVES The biocompatibility of dental casting alloys is a critical issue because these alloys are in long-term intimate contact with oral tissues. Since the biocompatibility of alloys is not completely known; the release of elements from the alloys has been studied. The aim of this study was to compare the elemental release from dental casting alloy during exposure to artificial saliva and ...

introduction: determination of best culture medium for fertilization and development of nmri mice. material and methods: development of fertilized mature oocytes of nmri mice was studied for five days in several culture media (ksom, t6, m16, czb, g1tm ver3 and g2tm ver3) following hormone therapy with pmsg and hcg. results: the rate of fertilization after 24 hours was 97.4% in ksom, 83.5% in t6...

background: this study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of nannochloropsis oculata. methods: culture of microalgae was done separately in walne, f/2, sato, and tmrl media. in the logarithmic and stationary growth phases, biomass production and lipid accumulat...

conclusions according to the results of the present study, it can be concluded that adipose derived mscs can be cultured in serum-free media with no change in their differentiating capacity. this finding gives us a hope for future cell therapy studies and trials with little concern about zoonotic infections or immunological reaction. results the isolated cells showed adherent spindle shaped mor...

BACKGROUND Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells should be investigated in order to avoid additional damage to the pulp tissue. This study evaluated the cytotoxicity of conventional and resin-modified glass ionom...

Recombinant human growth hormone (r-hGH) can be produced by recombinant Escherichia coli a main host for recombinant protein productions. In order to improve the productivity of r-hGH, an orthogonal (L 16 (4×2)) experiment was applied to optimize the best culture conditions for r-hGH production by flask cultures of E. coli BL (21) harboring a new constructed plasmid (pEHUb-hGH). Based on the re...

Conventional high-dose chemotherapy frequently leads to severe neutropenia, during which patients experience a high risk of infection. Although support care with donor's neutrophils is possible this choice is largely hampered by the limited availability of matched donors. To overcome this problem, we explored a large-scale ex vivo production of neutrophils from hematopoietic stem cells (HSCs) u...

Two commercial swab transport systems, Copan Amies gel agar with and without charcoal (Copan Diagnostics, Corona, Calif.), were compared to direct inoculation onto modified Thayer-Martin medium for detection of Neisseria gonorrhoeae in 1,490 endocervical specimens obtained from women attending a sexually transmitted disease clinic. Copan swabs were held in the transport system for 24 h at room ...

introduction: cyclosporin a (cya) is a potent immunosuppressive drug widely used in transplant settings due to its specific inhibition of t–cell activation. several studies have reported the synergistic effect of cya with hematopoietic growth factor in colony culture of mice bone marrow. we studied the effect of il-3, il-6, scf,cya and nutrition medium ( condition medium - pha) on the developme...