Microfluidic systems with integrated analysis and control of environmental parameters can enable precise control of metabolic activity and protein expression. Here, we couple microfluidic controls in a polycarbonate-PDMS microbioreactor (Fig. 1) with closed-loop control of temperature, pH, dissolved oxygen, and cell density [1,2] to the biochemical control of synthetic gene circuits [3,4]. In s...

A multicusp ion source with modular design was developed at LBNL for production of H− ions. The source consists of a front plate, two multicusp front chambers, a quartz flange with external 3-loop RF antenna and a rear multicusp chamber. The source has LaB6 sputtering target at the rear chamber to lower the work function of the surfaces by coating them with LaB6 and an external cesium oven on t...

PURPOSE The purpose of this study was to investigate the optimal molecular weight of dispersive sodium hyaluronate (Na-HA) for the reduction of corneal endothelial damage induced by sonication, irrigation, and aspiration, using enucleated pig eyes. METHOD The phaco-needle of phacoemulsification and aspiration (PEA) equipment was inserted into the anterior chamber after aqueous humor replaceme...

Intermittent-flow respirometry is an experimental protocol for measuring oxygen consumption in aquatic organisms that utilizes the best features of closed (stop-flow) and flow-through respirometry while eliminating (or at least reducing) some of their inherent problems. By interspersing short periods of closed-chamber oxygen consumption measurements with regular flush periods, accurate oxygen u...

We present an active droplet merging device, which can merge various sizes of micro droplets in different numbers by using pneumatically controlled horizontal PDMS microvalves. The merging part consists of a main and side channels separated by a pillar array. The pillar array structure is contained within a microfuidic channel. The function of the pillar array provides a bypass path to the cont...

Ozone-driven chemistry is a source of indoor secondary pollutants of potential health concern. This study investigates secondary air pollutants formed from reactions between constituents of household products and ozone. Gas-phase product emissions were introduced along with ozone at constant rates into a 198-L Teflon-lined reaction chamber. Gas-phase concentrations of reactive terpenoids and ox...

The exfoliated nanocomposite is of particular interest because it maximizes the interactions between polymers and clays, and thus it should result in dramatic improvements in mechanical and physical properties. However, it seems to be very difficult to exfoliate organoclay in epoxy resins. Organoclay is always aggregated on the micro-scale in epoxy resins with conventional dispersion methods. B...

Abstract This article is devoted to the investigation of characteristics a low-emission gas turbine combustion chamber, which can be used in Floating Production, Storage and Offloading (FPSO) vessels operates on mixture natural hydrogen. A new approach proposed for modelling processes burning out with hydrogen under preliminary mixing conditions gaseous fuel an oxidizer channels radial-axial sw...

This work reviews the current operational condition and activities on design modification for different applications of ejectors. Ejectors being a simple mechanical system capable performing multiple fluid related functions (vacuum generation, pumping, mixing, condensing heat exchanging), have been an essential part several industrial processes. Two areas emphasized; internal flow application-b...