Enhancing the endogenous capacity of myelin repair is a major therapeutic challenge in demyelinating diseases such as multiple sclerosis. We found that progesterone and the synthetic 19-norprogesterone derivative 16-methylene-17α-acetoxy-19-norpregn-4-ene-3,20-dione (Nestorone) promote the remyelination of axons by oligodendrocytes after lysolecithin-induced demyelination in organotypic culture...

Four experiments were conducted to evaluate lipid digestibility in finishing swine fed chemically hydrogenated fats. Dietary chromic oxide was used as an inert marker to measure the apparent digestibility of supplemental fat (SF) that consisted of fully hydrogenated (FH), partially hydrogenated (PH), or PH products blended with other fat sources. In Exp. 1, diets containing 5% SF (as-fed basis)...

It was previously proposed that the activation of rat liver phenylalanine hydroxylase (EC 1.14.16.1) by cAMP-dependent protein kinase-mediated phosphorylation of Ser-16 is due to the introduction of the negatively charged phosphate group. To explore the validity of this proposal, we have applied site-directed mutagenesis to specifically replace Ser-16 with negatively charged amino acids, glutam...

The role of phospholipid in rat liver microsomal glucose 6phosphatase has been investigated with the use of phospholipase A and phospholipase C to alter the microsomal phospholipids. The phospholipase C-treated preparation lost a maximum of 80 to 90% of the original activity, and 70% of the microsomal phospholipid was hydrolyzed. Addition of phospholipid completely restored the original activit...

1. The properties of rat brain lysophospholipase were investigated. Three subcellular fractions were employed: a particulate preparation which sedimented at 26,000 X g, a microsomal preparation which sedimented at 100,000 X g, and the supernatant of the above. The soluble enzyme represented only a small fraction of the total activity; it was further purified by treatment with protamine and ammo...

A soluble adenosine triphosphatase was obtained from skeletal muscle microsomes by extraction with deoxycholate. The Mg++-moderated soluble microsomal ATPase is activated loto ZO-fold by 10m5 M Gaff and inhibited by ethylene glycol bis(P-aminoethyl ether)tetraacetate. Spontaneous formation of microsomal vesicles from solubilized microsomes occurs on removal of deoxycholate by dilution with wate...