C1-inhibitor is increasingly used experimentally and clinically in inflammatory conditions like septicemia and ischemia-reperfusion injury. Several mechanisms may account for the anti-inflammatory effects of C1-inhibitor, including inhibition of complement. The aim of the present study was to investigate and compare the supraphysiologic effect of C1-inhibitor on the three complement pathways. N...

We recently identified ABO(H) blood group structures in Asn-linked sugar chains of human von Willebrand factor (vWF) purified from factor Vlll concentrates (J Biol Chem 267:8723, 1992). We surveyed plasma glycoproteins carrying ABO(H) blood group antigens by Western blotting analysis and sandwich enzyme-linked immunosorbent assay using blood group-specific monoclonal antibodies (MoAbs) and a le...

The adhesion of rhizobia to surfaces of clover roots was examined by an indirect plate-counting assay and phase-contrast microscopy. The number of Rhizobium trifolii cells attached to clover root segments increased in approximately linear fashion during the first hour of incubation, but did not change appreciably thereafter. The addition of 30 mM-2-deoxy-D-glucose, which effectively inhibits bi...

Recent biochemical and genetic evidence has shown that the endogenous lectin discoidin-I is involved in intercellular adhesion during development of the cellular slime mold Dictyostelium discoideum. We have prepared discoidin-I affinity columns and used them to isolate the lectin receptors. By using the cell surface radioiodination method, 11 discoidin-I binding proteins were identified in wild...

Objective: Potato (Solanum tuberosum) and garlic (Allium sativum) are an important ubiquitously consumed dietary components and known to contain lectins, with potent and diverse biological functions. Present study aims to evaluate the mitogenicity and immunomodulatory potential of these lectins on human and murine lymphocytes. Methods: The lectin activity of raw and heat processed extracts alon...

BACKGROUND/AIMS Compared with normal colonic mucosa, lectin receptor expression is increased in hyperplastic and neoplastic tissues; some lectins have been shown to influence human colonic epithelial cell proliferation. The aim was to assess further the influence of five lectins (Phaseolus vulgaris (PNA), Griffonia simplicifolia (GSA), concanavalin A (Con A), wheat germ (WGA), and peanut (PHA-L...

Proteins are polymers of amino acids (molecules containing an amino group, a carboxylic group and a hydrophobic or hydrophilic side chain) present in all organisms. Apolar, polar uncharged and electrically charged amino acids are covalently linked through peptide bonds (amide bonds) and the sequence they form in the polypeptide chain (primary structure) determines the tertiary or quaternary str...

Background and objective: Chronic kidney disease represents a leading cause of death in many countries. End-stage renal is the final stage chronic disease, which kidneys are no longer function well enough for patient to live without replacement therapy. Galectin-3 soluble beta-galactoside-binding lectin protein involved fibrosis solid organs, including heart kidney. This study aimed evaluate ga...

Abstract Siglec-8, a lectin involved in the suppression of allergic responses, is considered therapeutic target for developing glycan materials that enable regulation inflammatory diseases. In such materials, “multivalent effect” essential regulating immune responses. The dramatically increases apparent affinities (avidities) glycan-lectin interactions by clustering ligand multivalently. Numero...

Endogenous lactose-binding proteins from adult chicken liver and intestine have been purified to homogeneity by affinity chromatography on asialofetuinderivatized Sepharose, followed by isoelectric focusing. Since these carbohydrate-binding proteins are assayed as hemagglutinins, they are referred to by the operational term lectins. Although the hemagglutination activity of these lectins is inh...