Isolated islets of ACI donor rats were cultured for 4 days at 37 degrees C and then grafted into male diabetic Wistar-Lewis rats into three different organ sites without adjuvant immunosuppression. None of 6 recipients of the intraportal injection of 10 islets per gram of body weight became normoglycemic. Similarly, six rats that received islets injected under the renal capsule remained diabeti...

Aims. To evaluate the antidiabetic properties of borapetol B known as compound 1 (C1) isolated from Tinospora crispa in normoglycemic control Wistar (W) and spontaneously type 2 diabetic Goto-Kakizaki (GK) rats. Methods. The effect of C1 on blood glucose and plasma insulin was assessed by an oral glucose tolerance test. The effect of C1 on insulin secretion was assessed by batch incubation and ...

Diet induced obese (DIO) mice can be stratified according to their weight gain in response to high fat diet as low responders (LDR) and high responders (HDR). This allows the study of β-cell failure and the transitions to prediabetes (LDR) and early diabetes (HDR). C57BL/6N mice were fed for 8 weeks with a normal chow diet (ND) or a high fat diet and stratified as LDR and HDR. Freshly isolated ...

No information is available on the rate of blood flow in transplanted islets. In this study, adult rats were partially depancreatized, and islets from the excised pancreas were then isolated, maintained for 7 d in tissue culture, and subsequently transplanted back to the animal, beneath the renal capsule. Some rats were rendered diabetic with streptozotocin before transplantation. A month after...

Translational research is necessary for the development of efficient experimental animal models that can be used to develop innovative medical treatments, such as improvements in organ or tissue transplantation. We have developed animal models that produce photogenic proteins in their islet cells: rats models expressing the gene for luciferase or green fluorescent protein (GFP), and pig models ...

Islets from male B10.BR mice (H-2) were isolated by the collagenase technique, handpicked with a Pasteur pipette, and incubated (either intact or after dispersion with Dispase) for 0, 3, 5, 7,10, or 14 days in tissue culture medium supplemented with either lymphokine supernatants or recombinant murine interferon-7. Islets and single cells were examined for IA molecules by use of indirect immuno...

Rats with diabetes induced by streptozotocin (STZ) and nicotinamide (NA) are often used in animal studies concerning various aspects of diabetes. In this experimental model, the severity of diabetes is different depending on doses of STZ and NA. Moreover, diabetic changes in rats with STZ-NA-induced diabetes are not fully characterized. In our present study, metabolic changes and insulin secret...

Leukocyte 12-lipoxygenase (12-LO) gene expression in pancreatic beta cells is upregulated by cytotoxic cytokines like IL-1beta. Recent studies have demonstrated that 12-LO inhibitors can prevent glutamate-induced neuronal cell death when intracellular glutathione stores are depleted. Therefore, 12-LO pathway inhibition may prevent beta-cell cytotoxicity. To evaluate the role of 12-LO gene expre...

Xu J, Zhang L, Chou A, Allaby T, Bélanger G, Radziuk J, Jasmin BJ, Miki T, Seino S, Renaud J-M. KATP channel-deficient pancreatic -cells are streptozotocin resistant because of lower GLUT2 activity. Am J Physiol Endocrinol Metab 294: E326–E335, 2008. First published November 27, 2007; doi:10.1152/ajpendo.00296.2007.—In wild-type mice, a single injection of streptozotocin (STZ, 200 mg/kg body wt...

When fed a high-energy (HE) diet, diabetes-prone (DP) Psammomys obesus develop type 2 diabetes with altered glucose-stimulated insulin secretion (GSIS). Beta-cell stimulus-secretion coupling was investigated in islets isolated from DP P. obesus fed a low-energy (LE) diet (DP-LE) and after 5 days on a HE diet (DP-HE). DP-LE islets cultured overnight in 5 mmol/l glucose displayed glucose dose-dep...