Systemic cobalt (Co) and chromium (Cr) concentrations may be elevated in patients with metal joint replacement prostheses. Several studies have highlighted the detrimental effects of this exposure on bone cells vitro, but underlying mechanisms remain unclear. In study, we use whole-genome microarrays to comprehensively assess gene expression primary human osteoblasts, osteoclast precursors matu...

Extracellular, intracellular or surface proteins can be used as putative markers to characterize human mesenchymal stem cells (hMSC). However, these markers are also expressed by other cell types and primary cell pools reveal considerable heterogeneity. Therefore, the simultaneous detection of several markers on a single cell appears to be an attractive approach to identify hMSC. Here we demons...

Zinc is an essential element with important role in stimulating the osteogenesis and mineralization suppressing osteoclast differentiation. In this study, new bioactive ZnCl 2 -doped sol-gel materials were designed to be applied as coatings onto titanium. The biomaterials physicochemically characterized cellular responses evaluated vitro using MC3T3-E1 osteoblasts RAW264.7 macrophages. effect o...

background: the mechanism of cadmium induced osteoporosis is not well understood, so in this study, we examined the toxicity of bone marrow mesenchymal stem cell (mscs) following treatment of rats with cdcl2 in drinking water, to revile the effect of this chemical on differentiation potential of mscs. methods: at the end of third passage, mscs were grown in the osteogenic medium for 21 days. to...

background periodontium may be able to respond to injuries by regeneration via the function of stem cells. objectives this study sought to assess the differentiation of human periodontal ligament stem cells (pdlscs) into osteoblasts in standard osteogenic medium and in a medium supplemented with 1,25-dihydroxyvitamin d3 (calcitriol). materials and methods in this experimental study, pdlscs were...

BACKGROUND AND PURPOSE A linkage between the neurotransmitter alpha-calcitonin gene-related peptide (alpha-CGRP) and particle-induced osteolysis has been shown previously. The suggested osteoprotective influence of alpha-CGRP on the catabolic effects of ultra-high molecular weight polyethylene (UHMWPE) particles is analyzed in this study in primary human osteoblasts. METHODS Primary human ost...

Bone is a dynamic tissue that is continuously being remodeled throughout life. Specialized cells called osteoclasts transiently break down old bone (resorption process) at multiple sites as other cells known as osteoblasts are replacing it with new tissue (bone formation). Usually, both resorption and formation processes are in balance and thereby maintain skeletal strength and integrity. This ...

Treatment with dexamethasone in human osteoblasts leads to oxidative stress and cell injures. NF-E2-related factor 2 (Nrf2) is a key anti-oxidant signaling. We want to induce Nrf2 activation via microRNA-mediated silencing its suppressor Keap1. Our results show that microRNA-200a ("miR-200a") expression depleted Keap1, causing Nrf2 protein stabilization in OB-6 osteoblastic cells. Reversely, th...

OBJECTIVE To assess the effects of epigallocatechin-3-gallate (EGCG) on cytokine-induced Cyr61 synthesis in human osteoblastic cells and the associated signalling pathways. The therapeutic effect of EGCG on CIA in rats was also studied. METHODS The expression of Cyr61 and NF-κB pathway molecules was examined by western blotting. CCL2 expression was assessed by northern blotting and ELISA. Int...