BACKGROUND Our laboratory has demonstrated previously that human tonsillar B lymphocytes express IL-13 mRNA OBJECTIVE: We sought to investigate IL-13 production by human B cells and the association between B cell-derived IL-13 and IgE secretion. METHODS Human B lymphocytes were isolated from tonsils and purified by means of rosetting with sheep RBCs or positive or negative selection with magn...

In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated the methylene chloride/methanol extract heartwood. They tested for in vitro protective effect against chromosome aberrations peripheral human lymphocytes, using cytokinesis-block micronucleus assay. All compounds (in minimal doses 1 μg/mL) exerted a beneficial by decreasing DNA damage lymphoc...

This report describes the inhibition of human mixed lymphocyte culture (MLC) reactions by rabbit antisera to intact and detergent solubilized, fractionated, human trophoblast membranes. Heat-inactivated antisera were passed through solid-phase immunoabsorption columns of normal human serum and extensively absorbed with human erythrocytes, lymphocytes and liver powder. Immunohistological experim...

Using PH]thymidine incorporation into DNA of human lymphocytes in culture, we have shown that human serum contains an ultrafiltrable factor(s) that sensitizes the activation of lymphocytes by phytohaemagglutinin. This (these) factor(s) can replace either human serum or foetal calf serum in the culture. In addition, it permits the expression, and therefore the assay, of human somatomedin A pepti...