This investigation addressed the role of major histocompatibility complex-encoded class I molecules in the activation and proliferation of human lymphocytes. We studied the effect of antibodies specific for HLA-A and HLA-B locus gene products on mitogen-stimulated peripheral blood mononuclear cell (PBMC) subpopulations. Three individually derived, well-characterized anti-HLA class I monoclonal ...

Loss or down-regulation of HLA class I antigens in tumor cells has been frequently observed in a variety of human malignancies and it represents an important cancer immune escape mechanism (Garrido et al. 1997a ; Marincola et al. 2000 ; Campoli et al. 2002 ; Chang et al. 2005 ; Aptsiauri et al. 2007 ). Viruses use similar mechanism to avoid recognition and elimination by the immune system (Ploe...

Aims: The frequencies of alloantibodies to platelet glycoproteins and HLA class 1 have not been reported in this part of the world. To determine the prevalence of these antibodies in Nigeria. Methods: The sera of 100 multiparous women were tested for antibodies to HPA and HLA class 1 antigens using GTI PakPlus solid phase ELISA Kit in a cross sectional study. Results: The prevalence of platelet...

BACKGROUND Anti-HLA antibodies and especially donor-specific antibodies (DSA) play a significant role in graft survival after solid organ transplantation. Their impact on long-term survival in adult liver transplantation (LT) is controversial, but they may be a risk factor. The effects of DSA after pediatric LT are still unclear. METHODS We performed a retrospective evaluation of DSA in sera ...

Cell surface HLA-I molecules (Face-1) consist of a polypeptide heavy chain (HC) with two groove domains (G domain) and one constant domain (C-domain) as well light chain, B2-microglobulin (B2m). However, HCs can also independently emerge unfolded on the cell without peptides B2m-free HC monomers (Face-2), homodimers (Face 3), heterodimers (Face-4). The transport these HLA variants from ER to wa...

background: it has been arguedthat unprecedented degree of human leukocyte antigen (hla) loci polymorphism within a population is requiredto avoid the devastating effects of infectious diseases. the present study was conducted to determine the associations between some of hla classi genes and the outcome of hepatitis b virus (hbv) infection.   methods: using sequential sampling method, 64 indiv...

AIMS To investigate the occurrence of abnormal patterns of HLA-ABC and HLA-DR expression in enteropathy associated T cell lymphoma and to relate such abnormalities to the Epstein Barr virus (EBV) status of the tumours. METHODS Eleven enteropathy associated T cell lymphomas were immunostained with HC10 (HLA-ABC heavy chain) and TAL 1B5 (HLA-DR alpha chain) monoclonal antibodies and polyclonal ...

We investigated the incidence of circulating corneal epithelium antibodies in patients with uveitis. A high percentage of the patients (42%) were positive, whereas only 4% of controls had these antibodies in their serum. Significantly more patients with anterior and diffuse uveitis had corneal epithelium antibodies than did those with posterior uveitis. Subdivision of anterior uveitis into HLA-...

The influence of antibodies against HLA-DP antigens detected with solid-phase assays on graft survival after kidney transplantation (KT) is uncertain. We evaluated with Luminex® the prevalence of pre- and posttransplant DP antibodies in 440 KT patients and their impact on graft survival. For 291 patients with available pretransplant samples, DP antibodies were present in 39.7% KT with pretransp...

The HLA region is considered to be the main genetic risk factor for rheumatoid arthritis. Previous research demonstrated that HLA-DRB1 alleles encoding the shared epitope are specific for disease that is characterized by antibodies to cyclic citrullinated peptides (anti-CCP). In the present study, we incorporated the shared epitope and either anti-CCP antibodies or rheumatoid factor into linkag...