نتایج جستجو برای: guanidine

تعداد نتایج: 3719  

Journal: :Journal of the American Chemical Society 2009
Kevin P Kent Luke M Oltrogge Steven G Boxer

Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncated GFPs is low, and the chromophore is either partially formed or not formed. An alternative method is presented in which full-length proteins are produced recombinantly with a protease site inserte...

Journal: :Journal of the American Chemical Society 2004
Laura B Sagle Jörg Zimmermann Philip E Dawson Floyd E Romesberg

Protein folding is a central problem in the biological sciences. To generate residue-specific information on the equilibrium folding of cytochrome c, we have semisynthesized the protein with specifically deuterated residues. The C-D bonds may be easily visualized in an otherwise transparent region of the IR spectra, even at high protein and denaturant concentrations. Plotted as a function of ad...

Journal: :The Journal of biological chemistry 2008
Ruchira Engel Adrie H Westphal Daphne H E W Huberts Sanne M Nabuurs Simon Lindhoud Antonie J W G Visser Carlo P M van Mierlo

To understand how proteins fold in vivo, it is important to investigate the effects of macromolecular crowding on protein folding. Here, the influence of crowding on in vitro apoflavodoxin folding, which involves a relatively stable off-pathway intermediate with molten globule characteristics, is reported. To mimic crowded conditions in cells, dextran 20 at 30% (w/v) is used, and its effects ar...

2003
S. P. Chopade

G-100 is used for higher molecular mass proteins. The solutions of proteins are prepared in guanidine hydrochloride (10 mg mL ), and cytochromec (10 mg mL ) is used as an internal standard. Descending chromatography is carried out at room temperature under an inclination angle of 253 to the horizontal. After 3 h of development the quotient Rs protein/Rs cytochrome c is calculated for each prote...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1976
J R Garel B T Nall R L Baldwin

The kinetics of the refolding reaction of ribonuclease A from high concentrations of guanidine hydrochloride or urea are biphasic, and show two refolding reactions whose rates differ 450-fold at pH 5.8 and 25 degrees. Measurements of cytidine 2'-phosphate binding during refolding, after stopped-flow dilution of guanidine hydrochloride (Gdn.HCl) or urea, show that functional bovine pancreatic ri...

Journal: :The Journal of Cell Biology 1984
T K Sampath A H Reddi

Subcutaneous implantation of coarse powders (74-420 micron) of demineralized diaphyseal bone matrix resulted in the local differentiation of endochondral bone. However, implantation of matrix with particle size of 44-74 micron (Fine matrix) did not induce bone. We have recently reported that the dissociative extraction of coarse matrix with 4 M guanidine HCl resulted in a complete loss of the a...

Journal: :Proceedings of the Japan Academy 1953

Journal: :The Keio Journal of Medicine 1961

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