Escherichia coli recovered from a nosocomially acquired urinary tract infection required 48 h of incubation on blood agar and did not grow on other routine clinical laboratory media. This bacterium dissociated readily into three colony types, all of which were confirmed as E. coli by DNA hybridization studies. Preliminary studies indicate a prolonged lag phase that could not be corrected by the...

Two corynefonn organisms (A 1082 and A 1062) isolated from the skin mucus of Arctic cod, are described in detail. Strain A1032 may be a new species; strain A 1062 is a strain of Corynebacterium erythrogenes. Three carotenoid pigments, all with very similar absorption spectra, were found in both strains. Two of these pigments were identified as neoxanthin and sarcinaxanthin. "he third pigment ha...

Clinical microbiology automation is currently limited by the lack of an in-plate culture identification system. Using an inexpensive, printed, disposable colorimetric sensor array (CSA) responsive to the volatiles emitted into plate headspace by microorganisms during growth, we report here that not only the presence but the species of bacteria growing in plate was identified before colonies are...

Five selective media for beta-haemolytic streptococci were tested and compared with the conventional blood agar plate using 200 throat swabs from children with possible streptococcal pharyngitis. The medium described by Liebermeister and Braveny, which is based on the reduction of nutrients and enhancement of the haemolytic activity of beta-streptococci, was markedly superior to the other selec...

AIM Manufacturers' instructions recommend changing the infusion line together with the infusion bottle after each administration. We investigated if the complete infusion line may be microbiologically contaminated after short-time antibiotic and rinse-solution application. METHOD Immediately after the change of an infusion administration set after 72 hours the remaining antibiotic solution wa...

*Corresponding Address: [email protected] The study was conducted to estimate the prevalence of subclinical mastitis (SCM) in dairy cows in the Bhaktapur District of Nepal. A total of 200 milk samples were collected from 50 dairy cows. The California mastitis test (CMT) was carried out on quarter milk samples to determine the prevalence of subclinical mastitis. California Mastitis Test (CMT)...

Rifampin is recommended for combination therapy of meningitis due to beta-lactam-resistant Streptococcus pneumoniae. High-level rifampin resistance (MIC, > or =4 mg/liter) has been mapped to point mutations in clusters I and III of rpoB of the pneumococcus. The molecular basis of low-level resistance (MICs, > or =0.5 and <4 mg/liter) was analyzed. Spontaneous mutants of clinical pneumococcal is...

We evaluated the Vitek GNI1 and Becton Dickinson Crystal E/NF identification systems for their ability to accurately identify 619 and 626 strains, respectively, of members of the family Enterobacteriaceae and other glucose-fermenting and non-glucose-fermenting gram-negative rods. All strains tested were taken from a stock collection and passed three times on 5% sheep blood agar prior to testing...

PURPOSE This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. MATERIALS AND METHODS Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses (Listerine®, Decapinol®, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP p...

The reported case is of primary pulmonary nocardiosis, caused by Nocardia brasiliensis, in a immunocompromised patient, which ended fatally despite appropriate treatment. The partially acid fast filamentous bacterium was predominant on direct examination of the sputum. It was cultured on blood agar, MacConkey agar and by paraffin baiting technique. The bacterium was resistant to cotrimoxazole, ...