نتایج جستجو برای: gene cassettes
تعداد نتایج: 1142012 فیلتر نتایج به سال:
Conditional gene targeting uses the insertion of expression cassettes for the selection of targeted embryonic stem cells. The presence of these cassettes in the final targeted chromosomal locus may affect the normal expression of the targeted gene and produce interesting knock down phenotypes. We show here that the selection cassette may then be selectively removed in vivo, using three appropri...
Modern plant breeding increasingly relies on genomic information to guide crop improvement. Although some genes are characterized, additional tools needed effectively identify and characterize associated with traits. To address this need, the mPing element from rice was modified serve as an activation tag induce expression of nearby genes. Embedding promoter sequences in resulted a decrease ove...
Findings: A total of 240 swab samples collected from the patient-contact and non-patient-contact surfaces of 80 X-ray cassettes were collected during four hospital visits. We found three X-ray cassettes (3.8%) had methicillin-resistant Staphylococcus aureus colonies isolated, and two other X-ray cassettes (2.5%) had methicillin-susceptible Staphylococcus aureus colonies isolated on their surfac...
s: The aadA genes, encoding resistance to streptomycin and spectinomycin, have been found as gene cassettes in different gram-negative and gram-positive bacterial species. The present report has revealed that the sequence of a new gene, aadA5, combining with the trimethoprim resistance gene dfr17 occured in a class 1integron. The integron was identified in a nosocomial pathogen Enterobacter clo...
Regulatable promoter systems allow gene expression to be tightly controlled in vivo. This is highly desirable for the development of safe, efficacious adenoviral vectors that can be used to treat human diseases in the clinic. Ideally, regulatable cassettes should have minimal gene expression in the "OFF" state, and expression should quickly reach therapeutic levels in the "ON" state. In additio...
BACKGROUND The direct isolation of integron gene cassettes from cultivated and environmental microbial sources allows an assessment of the impact of the integron/gene cassette system on the emergence of new phenotypes, such as drug resistance or virulence. A structural approach is being exploited to investigate the modularity and function of novel integron gene cassettes. METHODOLOGY/PRINCIPA...
Integrons are powerful bacterial genetic elements that permit the expression and dissemination of antibiotic-resistance gene cassettes. They contain a promoter Pc that allows the expression of gene cassettes captured through site-specific recombination catalyzed by IntI, the integron-encoded integrase. Class 1 and 2 integrons are found in both clinical and environmental settings. The regulation...
Seventy-two isolates representing 18 serotypes recovered from various food samples collected in Colombia were tested for antimicrobial susceptibilities. The collection was further characterized for extended-spectrum cephalosporin, aminoglycoside, and tetracycline resistance markers. Multidrug resistant (MDR) isolates were further investigated for class 1 integrons and were evaluated for the pre...
Isogenic settings are routine in model organisms, yet remain elusive for genetic experiments on human cells. We describe the use of designed zinc finger nucleases (ZFNs) for efficient transgenesis without drug selection into the PPP1R12C gene, a "safe harbor" locus known as AAVS1. ZFNs enable targeted transgenesis at a frequency of up to 15% following transient transfection of both transformed ...
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