We describe an immunofluorometric assay for human pancreatic phospholipase A2 based on time-resolved fluorescence. The labeled antibody technique in combination with the time-resolved 1-s fluorometric detection of the europium label, which essentially eliminates all background fluorescence, resulted in a high sensitivity (20 ng/L) and a wide (5000-fold) linear range. Nonspecific binding was min...

An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several β-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay...

To separate the MM and MB creatine kinase (EC 2.7.3.2) isoenzymes in small plasma samples, we developed a simple, rapid ion-exchange batch-adsorption procedure with "DEAE Glycophase" glass beads (Corning). All separative steps are performed in a single test tube and can be completed within a few minutes. Results of measurements of isoenzymes in plasma samples from patients with acute myocardial...

The beta-glucuronidase reporter gene has been used to develop a sensitive assay for the excision of transposable elements introduced into transgenic plants. The reporter gene, inactivated by the insertion of the maize transposable element Activator (Ac) into the 5'-untranslated leader, was introduced into the genome of tobacco by Agrobacterium-mediated transformation. Reactivation of the beta-g...

We describe an immunoassay for human pregnancyspecific f31-glycoprotein by use of fluorescein-labeled antibodies to this protein. The technique is generally similar to that of a “two-site” immunoradiometric assay but has the advantage of stability of tracer and avoidance of radiation hazard. However, the procedure requires relatively large quantities of antibody. Furthermore, the within-assay c...

Three experiments were conducted to develop a sensitive and reliable fluorometric thiobarbituric acid (TBA) reactive substances (TBARS) method to determine lipid oxidation products in meat. The first study was conducted to find the optimum sample preparation conditions for meat in the fluorometric method. The second study was to compare the original and the modified methods by using meat and bl...

Accurate DNA quantification is a highly important method within molecular biology. Methods widely used to quantify are UV spectrometry and fluorometry. In this research, seven different samples one blank (MilliQ ultrapure water) were quantified by three analysts using spectrophotometric (i.e., NanoDrop instrument) fluorometric the AccuGreen High Sensitivity kit, AccuClear Ultra Qubit dsDNA HS A...