Abstract AIMS The kynurenine (Kyn) pathway plays an important role in the pathogenesis of many cancers including glioblastomas (GBMs). enzymes, indoleamine-2,3-dioxygenase (IDO1) and tryptophan-2,3-dioxygenase (TDO2), regulate first rate-limiting step resulting formation N-formyl-kynurenine, which is then readily converted into Kyn by formaldehyde. Cancers expressing IDO1 TDO2 enzymes can produ...

Inflammation is associated with the development of several diseases comprising cancer and cardiovascular disease. Agents that suppress cyclooxygenase (COX) lipoxygenase (LOX) enzymes, besides chemokines have been suggested to minimise inflammation. Here, a variety novel heterocyclic non-heterocyclic compounds were prepared from three furanone derivatives. The structures all synthesised confirme...

Metabolite genome-wide association studies (mGWASs) are increasingly used to discover the genetic basis of target phenotypes in plants such as Populus trichocarpa , a biofuel feedstock and model woody plant species. Despite their growing importance genetics metabolomics, few mGWASs experimentally validated. Here, we present functional genomics workflow for validating mGWAS-predicted enzyme–subs...

Assays of cortisol-metabolizing enzymes in homogenates of human trabecular meshwork cells under optimal conditions revealed two defects in primary open-angle glaucoma (POAG): one is a marked increase in delta 4-reductase and the other is a decrease in 3-oxidoreductase. Experiments indicated that the differences in enzyme activities seen between POAG and nonPOAG trabecular meshwork derived cell ...

Trans-resveratrol, a phytoalexin produced by a variety of plants, has been shown to inhibit oxidative enzymes in an animal cell system. Its effect on several oxidative and antioxidative enzymes from plants was investigated using in vitro assays. Trans-resveratrol inhibited superoxide dismutase, lipoxygenase, catalase, peroxidase, polyphenol oxidase, and 1-aminocyclopropane-1-carboxylic acid oxi...

Background: Nitrase Therapeutics is unlocking the therapeutic potential of nitrases, a new class enzymes discovered by company that catalyze post-translation modification called nitration. Materials And Methods: Nitrases nitration, process which an NO2 group transferred from peroxynitrite to specific tyrosines on substrates (nitro-substrates). Nitration important modifier protein structure, fun...

Epoxide hydrolase and glutathione S-transferase activities toward trans- and cis-stilbene oxides were measured in 3 strains of Drosophila melanogaster. Differences in age dependence, substrate selectivity and subcellular location were detected suggesting the presence of multiple forms of these enzymes. In addition, interstrain differences indicate the presence of genetic variation for epoxide h...