for the evaluation of autoantibodies in pemphigus vulgaris (pv), an indirect elisa assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (m.w.) of 130-160 kda. the evaluation of pemphigus igg, igm and iga autoantibodies in 75 patients and 50 control sera indicated that this indirect elisa assay was a useful method for the detection of ...

background: patients with prolonged neutropenia and/or severe underlying immunosuppression are at thegreatest risk for disseminated aspergillosis. this study was undertaken to determine the incidence of invasiveaspergillosis by platelia aspergillus enzyme-linked immunosorbent assay elisa kit in high risk children admitted tothe hematology ward of dr. faghihi hospital, shiraz university of medic...

background: toxoplasmosis is a parasitic disease caused by protozoan, toxoplasma gondii. infections of human are common and are usually asymptomatic. the infection may be serious if is transmitted to the fetus during pregnancy. prophylactic measures, early detection of the infection and treatment can avoid congenital toxoplasmosis and many long term effects. objective: seroepidemiological study...

this study was conducted to evaluate and compare the levels of aflatoxin m1 in raw and pasteurized milk samples during different seasons by enzyme- linked immuno sorbent assay in sanandaj, iran. in 257 (94.49%) out of 272 milk samples the presence of aflatoxin m1 was detected in concentrations ranging between 0.007 and 115.930 ng/l. afm1 level in 12 (4.4%) of positive samples were higher than t...

background: enzyme linked immunosorbent assay (elisa) has been described as an alternative to radioimmunoassay for the mammalian and nonmammalian steroids detection. in this study, a simple and rapid elisa is described and validated for 4-pregnen-3,20, dione (progesterone). materials and methods: a general procedure for preparation of the acetylcholinesterase labelled steroid is described which...

background: toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. the classical diagnosis of toxoplasmosis relies on serological methods. detection of parasite or its components could be useful tool for early diag­nosis of the infection. methods: fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of t. gondii rh strain. five of them sacrifi...

OBJECTIVE There have been a large number of reports on alterations in the serum level of keratan sulphate (KS), a potential marker of articular cartilage degeneration in patients with arthropathy. Such studies have commonly employed ELISA using the anti-KS monoclonal antibody 1/20/5D4 (5D4-ELISA) to determine KS levels. Recently, a highly sensitive KS ELISA (HS-ELISA) kit has been developed, al...

background and aims: measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including iran. methods: the present study was performed to evaluate the immune status agai...