O ne way to attempt periodontal regeneration is to mimic the biologic processes involved in nascent tooth development, especially the development of the root and the supporting tissues. The EMDOGAIN® (BIORA AB, Malmö, Sweden) concept is based on the discovery that enamel matrix proteins are not only involved in enamel formation but also play a key role in the formation of acellular cementum [1,...

Various periodontal plastic surgical techniques are employed in obtaining root coverage. Recently, the use of an enamel matrix derivative (EMD) has been reported in such treatment. We report 2 cases of root coverage surgery with a coronally positioned flap in combination with EMD (CPF+EMD) and connective tissue graft in combination with EMD (CTG+EMD). Case 1: The patient was a 25-year-old woman...

Background—Periodontal disease results in the loss of the attachment apparatus. In the last three decades, an increasing effort has been placed on seeking procedures and materials to promote the regeneration of this tissue. The aim of this paper is to evaluate the effect of enamel matrix derivative (EMD) during regenerative procedures. In addition, a meta-analysis is presented regarding the cli...

To determine the shear bond strength of 10 self-etching adhesive systems to dentin and enamel, flat bonding sites were prepared on 216 extracted human molar teeth, using 600-grit silicon carbide paper to expose dentin or enamel. Following the application of each adhesive system, bonded assemblies of Spectrum TPH were prepared using a gelatin capsule matrix (n = 24). After 24 hours of storage in...

A primary goal of enamel research is to understand and potentially treat or prevent enamel defects related to amelogenesis imperfecta (AI). Rodents are ideal models to assist our understanding of how enamel is formed because they are easily genetically modified, and their continuously erupting incisors display all stages of enamel development and mineralization. While numerous methods have been...

We describe studies of the interaction of ≅400ns duration, multiline (2.6-3) μm HF laser with enamel tissue. The etch rate experiment showed an ablation threshold fluence of 47 Jcm but using the dispersion theory for optical coefficients of n and k for enamel at dominant laser lines, this value reduced to ≅25 Jcm making it more comparable with other investigations. The main mechanism of enamel ...

We have previously identified amelotin (AMTN) as a novel protein expressed predominantly during the late stages of dental enamel formation, but its role during amelogenesis remains to be determined. In this study we generated transgenic mice that produce AMTN under the amelogenin (Amel) gene promoter to study the effect of AMTN overexpression on enamel formation in vivo. The specific overexpres...

Dental enamel development occurs in stages. The ameloblast cell layer is adjacent to, and is responsible for, enamel formation. When rodent pre-ameloblasts become tall columnar secretory-stage ameloblasts, they secrete enamel matrix proteins, and the ameloblasts start moving in rows that slide by one another. This movement is necessary to form the characteristic decussating enamel prism pattern...

Tooth development is regulated by epithelial-mesenchymal interactions and their reciprocal molecular signaling. Bone morphogenetic protein 2 (Bmp2) is essential for tooth formation. However, the role of Bmp2 during enamel formation remains unknown in vivo. In this study, the role of Bmp2 in the regulation of postnatal enamel formation was investigated via the conditional ablation of Bmp2 in ena...